Structure of spinach chloroplast F1-ATPase complexed with the phytopathogenic inhibitor tentoxin

Proceedings of the National Academy of Sciences of the United States of America
Georg Groth

Abstract

Tentoxin, a natural cyclic tetrapeptide produced by phytopathogenic fungi from the Alternaria species affects the catalytic function of the chloroplast F(1)-ATPase in certain sensitive species of plants. In this study, we show that the uncompetitive inhibitor tentoxin binds to the alphabeta-interface of the chloroplast F(1)-ATPase in a cleft localized at betaAsp-83. Most of the binding site is located on the noncatalytic alpha-subunit. The crystal structure of the tentoxin-inhibited CF(1)-complex suggests that the inhibitor is hydrogen bonded to Asp-83 in the catalytic beta-subunit but forms hydrophobic contacts with residues Ile-63, Leu-65, Val-75, Tyr-237, Leu-238, and Met-274 in the adjacent alpha-subunit. Except for minor changes around the tentoxin-binding site, the structure of the chloroplast alpha(3)beta(3)-core complex is the same as that determined with the native chloroplast ATPase. Tentoxin seems to act by inhibiting inter-subunit contacts at the alphabeta-interface and by blocking the interconversion of binding sites in the catalytic mechanism.

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Citations

Jan 24, 2007·Nature Structural & Molecular Biology·Raz ZarivachNatalie C J Strynadka
May 9, 2009·The Journal of Biological Chemistry·Melanie VollmarGeorg Groth
Jun 27, 2008·The Journal of Biological Chemistry·Erik MeissToru Hisabori
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