Structure of T4moC, the Rieske-type ferredoxin component of toluene 4-monooxygenase

Acta Crystallographica. Section D, Biological Crystallography
Luke A MoeBrian G Fox

Abstract

The structure of the Rieske-type ferredoxin (T4moC) from toluene 4-monooxygenase was determined by X-ray crystallography in the [2Fe-2S](2+) state at a resolution of 1.48 A using single-wavelength anomalous dispersion phasing with the [2Fe-2S] center. The structure consists of ten beta-strands arranged into the three antiparallel beta-sheet topology observed in all Rieske proteins. Trp69 of T4moC is adjacent to the [2Fe-2S] centre, which displaces a loop containing the conserved Pro81 by approximately 8 A away from the [2Fe-2S] cluster compared with the Pro loop in the closest structural and functional homolog, the Rieske-type ferredoxin BphF from biphenyl dioxygenase. In addition, T4moC contains five hydrogen bonds to the [2Fe-2S] cluster compared with three hydrogen bonds in BphF. Moreover, the electrostatic surface of T4moC is distinct from that of BphF. These structural differences are identified as possible contributors to the evolutionary specialization of soluble Rieske-type ferredoxins between the diiron monooxygenases and cis-dihydrodiol-forming dioxygenases.

Citations

Apr 28, 2011·Applied Microbiology and Biotechnology·Patrizia Di GennaroGuido Sello
Aug 16, 2008·Acta Crystallographica. Section D, Biological Crystallography·Elena J LevinGeorge N Phillips
Jan 21, 2009·Acta Crystallographica. Section D, Biological Crystallography·Rosmarie FriemannS Ramaswamy
Jun 12, 2014·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Nicholas E KaragasLaura M Hunsicker-Wang
Aug 26, 2014·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Md Ehesan AliDominik Marx
Nov 18, 2014·Biochemistry·Alexandria Deliz Liang, Stephen J Lippard
Oct 30, 2007·Protein Expression and Purification·Lucas J BaileyBrian G Fox
Oct 15, 2013·Proteins·Thom VrevenZhiping Weng
Aug 27, 2015·Biochemistry·Justin F AchesonBrian G Fox
May 20, 2008·Advances in Applied Microbiology·R E ParalesJ L Ditty

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