Structure of the [2Fe-2S] ferredoxin I from the blue-green alga Aphanothece sacrum at 2.2 A resolution

Journal of Molecular Biology
T TsukiharaH Matsubara

Abstract

Crystals of a [2Fe-2S] ferredoxin (Fd) I with a relative molecular mass of 10,480 were obtained from the blue-green alga Aphanothece sacrum. Each asymmetric unit of the crystal contains four molecules. An electron density map calculated by the single isomorphous replacement method with the anomalous dispersion at 2.5 A resolution was refined by averaging the four molecules in the asymmetric unit. Positional and isotropic thermal parameters for the non-hydrogen atoms of the four molecules and 158 water molecules were refined to an R-factor (R = sigma[Fo-Fc[/sigma Fo) of 0.23 by the restrained least-squares method. The estimated root-mean-square (r.m.s.) error for the atomic positions is 0.3 A. The r.m.s. deviations of equivalent C alpha atoms of the asymmetric-unit molecules superposed by the least-squares method average 0.35 A. The Fd molecule has a structure like the beta-barrel in the molecule of the [2Fe-2S] Fd from Spirulina platensis. A [2Fe-2S] cluster is bonded covalently to the protein molecule by four Fe-S, in which three of the Fe-S bonds are in a loop segment from position 38 to 47. The hydrophobic core inside the beta-barrel is formed by seven conservative residues: Val15, Val18, Ile24, Leu51, Ile74, Ala79 and Ile87...Continue Reading

References

Oct 1, 1978·Journal of Biochemistry·A KunitaH Matsubara
Feb 1, 1976·Journal of Biochemistry·T HaseH Matsubara
Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E AdmanL H Jensen
Nov 1, 1974·Quarterly Reviews of Biophysics·R H Sands, W R Dunham
Mar 1, 1980·International Journal of Peptide and Protein Research·M Go, S Miyazawa

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Citations

Sep 1, 1996·Protein Science : a Publication of the Protein Society·M Vidakovic, J P Germanas
Feb 1, 1994·Journal of Bioenergetics and Biomembranes·H M HoldenJ L Markley
Feb 1, 1994·Journal of Bioenergetics and Biomembranes·P A Karplus, C M Bruns
Jun 8, 2002·Journal of Molecular Biology·Chetlen R CrossnoeKurt L Krause
Jun 24, 2003·Journal of Molecular Biology·Robert H H van den HeuvelAndrea Mattevi
Jan 1, 1996·Progress in Biophysics and Molecular Biology·C Herrmann, N Nassar
Oct 24, 1998·Progress in Biophysics and Molecular Biology·H Sticht, P Rösch
Aug 1, 1996·Nature Structural Biology·N NassarA Wittinghofer
Jul 1, 1999·Protein Science : a Publication of the Protein Society·A M WyndhamG Chelvanayagam
Jul 19, 2000·Proteins·A V GrinbergR Bernhardt
Dec 18, 2002·Annals of the New York Academy of Sciences·Nicolas RouhierJean-Pierre Jacquot
Dec 18, 2002·Annals of the New York Academy of Sciences·Jean-Pierre JacquotEric Gelhaye
Jul 9, 1998·Journal of Molecular Biology·P BayerJ Becker
Oct 15, 1993·Structure·C A Orengo, J M Thornton
Oct 1, 1992·Archives of Biochemistry and Biophysics·J KieleczawaG Hind
Nov 7, 1996·Chemical Reviews·Richard H. HolmEdward I. Solomon

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