Structure of the adenylation domain of NAD(+)-dependent DNA ligase from Staphylococcus aureus.

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Seungil HanMatt Griffor

Abstract

DNA ligase catalyzes phosphodiester-bond formation between immediately adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA and plays a central role in many cellular and biochemical processes, including DNA replication, repair and recombination. Bacterial NAD(+)-dependent DNA ligases have been extensively characterized as potential antibacterial targets because of their essentiality and their structural distinction from human ATP-dependent DNA ligases. The high-resolution structure of the adenylation domain of Staphylococcus aureus NAD(+)-dependent DNA ligase establishes the conserved domain architecture with other bacterial adenylation domains. Two apo crystal structures revealed that the active site possesses the preformed NAD(+)-binding pocket and the 'C2 tunnel' lined with hydrophobic residues: Leu80, Phe224, Leu287, Phe295 and Trp302. The C2 tunnel is unique to bacterial DNA ligases and the Leu80 side chain at the mouth of the tunnel points inside the tunnel and forms a narrow funnel in the S. aureus DNA ligase structure. Taken together with other DNA ligase structures, the S. aureus DNA ligase structure provides a basis for a more integrated understanding of substrate recognition and catalysis and will be a...Continue Reading

References

Jan 1, 1992·Annual Review of Biochemistry·T Lindahl, D E Barnes
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Aug 1, 1996·Nucleic Acids Research·J Luo, F Barany
Aug 26, 1998·The New England Journal of Medicine·F D Lowy
May 20, 1999·Nature Structural Biology·A PerrakisV S Lamzin
Jun 16, 1999·Structure·M R SingletonD B Wigley
Aug 18, 2000·Mutation Research·D J TimsonD B Wigley
Jul 10, 2001·Molecular Microbiology·A WilkinsonR Bowater
Jan 10, 2002·The Journal of Biological Chemistry·Verl Sriskanda, Stewart Shuman
Jul 18, 2003·The Journal of Biological Chemistry·Heike Brötz-OesterheltHarald Labischinski
Dec 8, 2004·Current Opinion in Structural Biology·Stewart Shuman, Christopher D Lima
Dec 17, 2005·Nature Reviews. Microbiology·E Yoko Furuya, Franklin D Lowy
Dec 20, 2005·Nucleic Acids Research·Sandeep Kumar SrivastavaRavishankar Ramachandran
Feb 9, 2006·Chemical Reviews·Alan E TomkinsonTom Ellenberger
May 1, 2007·Molecular Cell·Jayakrishnan NandakumarStewart Shuman
Dec 15, 2007·Medicinal Research Reviews·Namrata DwivediRama Pati Tripathi

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Citations

May 16, 2012·Antimicrobial Agents and Chemotherapy·Steven D PodosMichael J Pucci
Dec 30, 2010·Antimicrobial Agents and Chemotherapy·Scott D MillsSuzanne S Stokes
Mar 31, 2012·Expert Opinion on Drug Discovery·Gautam Sanyal, Peter Doig
Jan 11, 2012·ACS Chemical Biology·Sushmita D LahiriScott D Mills
Jun 6, 2014·ACS Medicinal Chemistry Letters·Steven HowardPamela A Williams

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