Mar 27, 2020

Structure of the ALS Mutation Target Annexin A11 Reveals a Stabilising N-Terminal Segment

BioRxiv : the Preprint Server for Biology
P. A. G. LillebostadS. J. Hjellbrekke


The functions of the annexin family of proteins involve binding to Ca2+, lipid membranes, other proteins, and RNA, and the annexins share a common folded core structure at the C terminus. Annexin A11 (AnxA11) has a long N-terminal region, which is predicted to be disordered, binds RNA, and forms membraneless organelles involved in neuronal transport. Mutations in AnxA11 have been linked to amyotrophic lateral sclerosis (ALS). We studied the structure and stability of AnxA11 and identified a short stabilising segment in the N-terminal end of the folded core, which links domains I and IV. Crystal structure of the AnxA11 core highlights main-chain hydrogen bonding interactions formed through this bridging segment, which are likely conserved in most annexins. The structure was also used to study the currently known ALS mutations in AnxA11. Three of these mutations correspond to buried Arg residues highly conserved in the annexin family, indicating central roles in annexin folding. The structural data provide starting points for detailed structure-function studies of both full-length AnxA11 and the disease variants being identified in ALS.

  • References
  • Citations


  • We're still populating references for this paper, please check back later.
  • References
  • Citations


  • This paper may not have been cited yet.

Mentioned in this Paper

Meta-Analysis (Publications)
Meta Analysis (Statistical Procedure)
Psychiatry Specialty
Gene Regulatory Networks
Statistical Technique

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Related Papers

Clinica Chimica Acta; International Journal of Clinical Chemistry
Jiasheng WangFrederick T Greenaway
Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Kelei HuaJianbin Gu
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences
Di LiaoFangfang Bi
© 2020 Meta ULC. All rights reserved