Structure of the bacterial RNA polymerase promoter specificity sigma subunit

Molecular Cell
Elizabeth A CampbellSeth A Darst

Abstract

The sigma subunit is the key regulator of bacterial transcription. Proteolysis of Thermus aquaticus sigma(A), which occurred in situ during crystallization, reveals three domains, sigma(2), sigma(3), and sigma(4), connected by flexible linkers. Crystal structures of each domain were determined, as well as of sigma(4) complexed with -35 element DNA. Exposed surfaces of each domain are important for RNA polymerase binding. Universally conserved residues important for -10 element recognition and melting lie on one face of sigma(2), while residues important for extended -10 recognition lie on sigma(3). Genetic studies correctly predicted that a helix-turn-helix motif in sigma(4) recognizes the -35 element but not the details of the protein-DNA interactions. Positive control mutants in sigma(4) cluster in two regions, positioned to interact with activators bound just upstream or downstream of the -35 element.

References

Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·L Johnsrud
Apr 15, 1976·European Journal of Biochemistry·P A Lowe, A D Malcolm
Jan 1, 1992·Annual Review of Biochemistry·C O Pabo, R T Sauer
Mar 11, 1992·Proceedings of the National Academy of Sciences of the United States of America·C H Jones, C P Moran
Jun 1, 1992·Journal of Bacteriology·M LonettoC A Gross
Aug 5, 1991·Journal of Molecular Biology·J MecsasC A Gross
Jun 11, 1991·Journal of Bacteriology·T J Kenney, C P Moran
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Feb 21, 1991·Journal of Theoretical Biology·R Paul, M Otwinowski
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D DanielsR Losick
Sep 20, 1990·Journal of Molecular Biology·C WaldburgerM M Susskind
Aug 1, 1988·Journal of Biomolecular Structure & Dynamics·R Lavery, H Sklenar
Apr 20, 1989·Journal of Molecular Biology·P ZuberR Losick
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T J KenneyC P Moran
Apr 20, 1989·Journal of Molecular Biology·T GardellaM M Susskind
Jan 1, 1988·Annual Review of Biochemistry·J D Helmann, M J Chamberlin
Aug 26, 1986·Nucleic Acids Research·M Gribskov, R R Burgess
Jan 4, 1969·Nature·R R BurgessE K Bautz
May 10, 1969·Nature·A A Travers, Burgessrr
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·U Siebenlist, W Gilbert
May 1, 1993·Journal of Bacteriology·A Ishihama
May 31, 1996·Journal of Molecular Biology·K MakinoM Suzuki
Aug 2, 1996·Science·L Holm, C Sander
Aug 27, 1996·Biochemistry·I BaikalovR E Dickerson
Nov 15, 1996·Journal of Molecular Biology·E SeverinovaS A Darst
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·D M JooR Calendar
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·P D AdamsA T Brünger
Aug 19, 1997·Proceedings of the National Academy of Sciences of the United States of America·P EichenbergerJ Geiselmann
Apr 17, 1998·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·H Nagai, N Shimamoto

❮ Previous
Next ❯

Citations

Mar 16, 2007·Plant Cell Reports·Eugene A Lysenko
Jun 14, 2005·Comptes rendus biologies·Sergei Borukhov, Jookyung Lee
Apr 6, 2005·FEMS Microbiology Reviews·L AravindLakshminarayan M Iyer
Dec 9, 2003·Journal of Molecular Biology·Marina ZakharovaKonstantin Severinov
Dec 23, 2003·Journal of Molecular Biology·Lars F WestbladeStephen J W Busby
Apr 6, 2004·Current Opinion in Microbiology·Sarah E Ades
Apr 23, 2004·Current Opinion in Structural Biology·Catherine L LawsonRichard H Ebright
Nov 29, 2002·Research in Microbiology·Sergei Borukhov, Konstantin Severinov
Feb 13, 2003·Current Opinion in Structural Biology·Katsuhiko S Murakami, Seth A Darst
May 7, 2003·Current Opinion in Microbiology·Sergei Borukhov, Evgeny Nudler
May 7, 2003·Current Opinion in Microbiology·David A SchneiderRichard L Gourse
May 18, 2013·Journal of Medicinal Chemistry·Vadim MolodtsovKatsuhiko S Murakami
Nov 6, 2007·Nature Methods·Aiping DongHaizhong Zhu
Jun 4, 2008·Nature Reviews. Microbiology·Shanil P HaugenRichard L Gourse
Jan 15, 2011·Nature Reviews. Microbiology·Finn Werner, Dina Grohmann
Apr 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·B D GregoryA Hochschild
Jan 1, 2005·Proceedings of the National Academy of Sciences of the United States of America·Caroline A DavisRuth M Saecker
Oct 8, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kumiko Sakata-Sogawa, Nobuo Shimamoto
Dec 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sergei NechaevE Peter Geiduschek
Mar 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Bianca SclaviHermann Heumann
Jan 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yvonne Berghöfer-HochheimerCarol A Gross
Aug 3, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ajit B DattaPradeep Parrack
Nov 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Margareta K Sorenson, Seth A Darst
Mar 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yongqing LiuMichael J Daly
Feb 7, 2008·Proceedings of the National Academy of Sciences of the United States of America·Daniel Klein-Marcuschamer, Gregory Stephanopoulos
Apr 16, 2009·Proceedings of the National Academy of Sciences of the United States of America·Andy H YuanAnn Hochschild
Sep 30, 2010·Proceedings of the National Academy of Sciences of the United States of America·Gilad OfekPeter D Kwong
Dec 3, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kelly-Anne F TwistSeth A Darst
May 3, 2012·Proceedings of the National Academy of Sciences of the United States of America·Sébastien CampagneJulia A Vorholt
Jul 17, 2013·Proceedings of the National Academy of Sciences of the United States of America·Devendra B SrivastavaElizabeth A Campbell
Jun 27, 2002·Proceedings of the National Academy of Sciences of the United States of America·Julio A CamareroTom W Muir
Dec 17, 2009·The Journal of Biological Chemistry·Swaroopa Paratkar, Smita S Patel
Aug 24, 2011·The Journal of Biological Chemistry·Muthiah KumaraswamiMartha M Howe
Sep 27, 2008·The Journal of Biological Chemistry·Patrick EnglandAnnie Kolb
Apr 12, 2003·Nucleic Acids Research·Norie FujikawaShigeyuki Yokoyama

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.