Structure of the capsular polysaccharide of the KPC-2-producing Klebsiella pneumoniae strain KK207-2 and assignment of the glycosyltransferases functions

International Journal of Biological Macromolecules
Barbara BellichPaola Cescutti

Abstract

Klebsiella pneumoniae strain KK207-2 was isolated in 2010 from a bloodstream infection of an inpatient at an Italian hospital. It was previously found to produce the KPC-2 carbapenemase and to belong to clade 1 of sequence type 258. Genotyping of the conserved wzi and wzc genes from strain KK207-2 yielded contrasting results: the wzc-based method assigned the cps207-2 to a new K-type, while the wzi-based method assigned it to the known K41 K-type. In order to resolve this contradiction, the capsular polysaccharide of K. pneumoniae KK207-2 was purified and its structure determined by using GLC-MS of appropriate carbohydrate derivatives, ESI-MS of both partial hydrolysis and Smith degradation derived oligosaccharides, and NMR spectroscopy of oligosaccharides, and the lithium degraded, native and de-O-acetylated polysaccharide. All the collected data demonstrated the following repeating unit for the K. pneumoniae KK207-2 capsular polysaccharide: The polysaccharide contains about 0.60 acetyl groups per repeating unit on C6 of the Gal residue. The reactions catalyzed by each glycosyltransferase in the cpsKK207-2 gene cluster were assigned on the basis of structural homology with other Klebsiella K antigens.

References

Oct 1, 1977·Carbohydrate Research·B LindbergJ Lönngren
Apr 1, 1986·Journal of Bacteriology·S F OsmanM L Fishman
Jul 17, 2013·Antimicrobial Agents and Chemotherapy·L S TzouvelekisG L Daikos
Oct 4, 2013·Journal of Clinical Microbiology·Sylvain BrisseDominique Decré
Dec 19, 2013·PloS One·Yi-Jiun PanJin-Town Wang
Mar 19, 2014·Proceedings of the National Academy of Sciences of the United States of America·Frank R DeleoBarry N Kreiswirth
Jun 17, 2016·Microbiology and Molecular Biology Reviews : MMBR·Michelle K Paczosa, Joan Mecsas

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