Structure of the complex of a yeast glucoamylase with acarbose reveals the presence of a raw starch binding site on the catalytic domain

The FEBS Journal
Jozef SevcíkKeith S Wilson

Abstract

Most glucoamylases (alpha-1,4-D-glucan glucohydrolase, EC 3.2.1.3) have structures consisting of both a catalytic and a starch binding domain. The structure of a glucoamylase from Saccharomycopsis fibuligera HUT 7212 (Glu), determined a few years ago, consists of a single catalytic domain. The structure of this enzyme with the resolution extended to 1.1 A and that of the enzyme-acarbose complex at 1.6 A resolution are presented here. The structure at atomic resolution, besides its high accuracy, shows clearly the influence of cryo-cooling, which is manifested in shrinkage of the molecule and lowering the volume of the unit cell. In the structure of the complex, two acarbose molecules are bound, one at the active site and the second at a site remote from the active site, curved around Tyr464 which resembles the inhibitor molecule in the 'sugar tongs' surface binding site in the structure of barley alpha-amylase isozyme 1 complexed with a thiomalto-oligosaccharide. Based on the close similarity in sequence of glucoamylase Glu, which does not degrade raw starch, to that of glucoamylase (Glm) from S. fibuligera IFO 0111, a raw starch-degrading enzyme, it is reasonable to expect the presence of the remote starch binding site at stru...Continue Reading

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Citations

Dec 15, 2010·Applied Microbiology and Biotechnology·Julia Marín-Navarro, Julio Polaina
Feb 9, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Jaeyong Lee, Mark Paetzel
Jul 16, 2013·Applied Microbiology and Biotechnology·Zilong LiNing Jiang
May 6, 2016·Cellular and Molecular Life Sciences : CMLS·Shane EmanuelleMatthew S Gentry
Aug 28, 2009·Critical Reviews in Biotechnology·Pardeep Kumar, T Satyanarayana
Jun 30, 2011·Critical Reviews in Biotechnology·Sven CuyversChristophe M Courtin
Sep 20, 2019·Biotechnology Advances·Štefan JanečekBirte Svensson

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