Structure of the DNA damage-inducible gene DDR48 and evidence for its role in mutagenesis in Saccharomyces cerevisiae.

Molecular and Cellular Biology
J M Treger, K McEntee

Abstract

The DDR48 gene of Saccharomyces cerevisiae is a member of a set of genes that displays increased transcription in response to treatments that produce DNA lesions or to heat-shock stress. Other members of this group include the DDRA2 and UBI4 genes. DNA sequence analysis of the DDR48 gene demonstrates the presence of two overlapping open reading frames, each of which has the capacity to encode a protein with a molecular mass of approximately 45 kilodaltons. Fusions of the DDR48 coding sequences to lacZ demonstrates that only one of these frames is expressed in yeast cells. The protein predicted from this sequence is extremely hydrophilic and contains multiple repeats of the peptide sequence Ser-Asn-Asn-X-Asp-Ser-Tyr-Gly where X is either Asn or Asp. Additionally, closely related sequences are found throughout the primary sequence. Primer extension data indicate that, after 4-nitroquinoline-1-oxide and heat-shock treatments, there are three major and two minor transcriptional start sites which are utilized. The function of the DDR48 gene was investigated by disrupting this gene in diploid cells. Viable haploid cells containing the DDR48 gene disruption were isolated after tetrad analysis. Although the ddr48 mutant showed a slight...Continue Reading

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Citations

Feb 18, 2011·The New Phytologist·Iris FischerWolfgang Stephan
Jul 11, 2006·Gene·Nicolás FrankelNorberto D Iusem
Jul 16, 2014·Journal of Biotechnology·Franziska BühligenSusann Müller
Dec 6, 2005·Molecular Genetics and Genomics : MGG·Caroline DubacqCarl Mann
Apr 24, 2012·Eukaryotic Cell·I A ClearyD P Thomas
Jun 1, 2002·Applied and Environmental Microbiology·Sonia Rodriguez-VargasFrancisca Randez-Gil
Sep 21, 2011·The Journal of Biological Chemistry·Brandon M HallKeshav K Singh

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