Structure of the DNA duplex d(ATTAAT)2 with Hoogsteen hydrogen bonds

PloS One
Francisco J Acosta-ReyesJ Lourdes Campos

Abstract

The traditional Watson-Crick base pairs in DNA may occasionally adopt a Hoogsteen conformation, with a different organization of hydrogen bonds. Previous crystal structures have shown that the Hoogsteen conformation is favored in alternating AT sequences of DNA. Here we present new data for a different sequence, d(ATTAAT)2, which is also found in the Hoogsteen conformation. Thus we demonstrate that other all-AT sequences of DNA with a different sequence may be found in the Hoogsteen conformation. We conclude that any all-AT sequence might acquire this conformation under appropriate conditions. We also compare the detailed features of DNA in either the Hoogsteen or Watson-Crick conformations.

References

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Citations

Oct 31, 2019·Nucleic Acids Research·Jocelyne VreedeDavid W H Swenson
Mar 23, 2018·Beilstein Journal of Organic Chemistry·Jens Müller
Nov 6, 2020·Scientific Reports·A Ben ImeddoureneO Mauffret
Jul 9, 2021·The Journal of Physical Chemistry. B·Robert J FickAllison L Stelling
Dec 15, 2017·The Journal of Physical Chemistry Letters·Debayan Chakraborty, David J Wales

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Methods Mentioned

BETA
X-ray
gel filtration

Software Mentioned

Phaser
XDS
Xscale
Refmac5
Refmac

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