PMID: 9448265Mar 14, 1998Paper

Structure of the elongating ribosome: arrangement of the two tRNAs before and after translocation

Proceedings of the National Academy of Sciences of the United States of America
K H NierhausH B Stuhrmann

Abstract

The ribosome uses tRNAs to translate the genetic information into the amino acid sequence of proteins. The mass ratio of a tRNA to the ribosome is in the order of 1:100; because of this unfavorable value it was not possible until now to determine the location of tRNAs within the ribosome by neutron-scattering techniques. However, the new technique of proton-spin contrast-variation improves the signal-to-noise ratio by more than one order of magnitude, thus enabling the direct determination of protonated tRNAs within a deuterated ribosome for the first time. Here we analyze a pair of ribosomal complexes being either in the pre- or post-translocational states that represent the main states of the elongating ribosome. Both complexes were derived from one preparation. The orientation of both tRNAs within the ribosome and their mutual arrangement are determined by using an electron microscopy model for the Escherichia coli ribosome and the tRNA structure. The mass center of gravity of the (tRNA)2mRNA complex moves within the ribosome by 12 +/- 4 A in the course of translocation as previously reported. The main results of the present analysis are that the mutual arrangement of the two tRNAs does not change on translocation and that t...Continue Reading

References

Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D Smith, M Yarus
Aug 16, 1974·Nature·J D RobertusA Klug
Jun 1, 1995·European Journal of Biochemistry·R Brimacombe
Nov 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·J FrankS Srivastava
Nov 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·K H NierhausR Jünemann
Sep 13, 1996·Journal of Molecular Biology·K R LataJ Frank
Oct 1, 1964·Journal of Molecular Biology·R R TRAUT, R E MONRO

❮ Previous
Next ❯

Citations

Feb 13, 2003·Journal of Molecular Biology·Gregor BlahaKnud H Nierhaus
Jul 27, 2001·Biochimica Et Biophysica Acta·R WillumeitK H Nierhaus
Feb 19, 2000·Current Opinion in Biotechnology·O Byron, R J Gilbert
Nov 8, 2012·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Feng-Biao GuoWen Wei
Aug 7, 2009·Journal of the Royal Society, Interface·Jeremy H Lakey
May 10, 2001·Protein Science : a Publication of the Protein Society·D BrockwellJ Barber
Apr 13, 2000·Annual Review of Entomology·G C YenchoP F Byrne
Nov 1, 2005·Journal of Theoretical Biology·Xu-Qing LiJinghong Fan
May 23, 1998·Critical Reviews in Biochemistry and Molecular Biology·N BurkhardtK H Nierhaus
Nov 26, 1998·The Journal of Biological Chemistry·M DabrowskiK H Nierhaus
Aug 15, 1998·Biological Chemistry·C M Spahn, K H Nierhaus
Dec 15, 2000·Journal of Molecular Biology·R WillumeitK H Nierhaus

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.