Structure of the EntB multidomain nonribosomal peptide synthetase and functional analysis of its interaction with the EntE adenylation domain.

Chemistry & Biology
Eric J DrakeAndrew M Gulick

Abstract

Nonribosomal peptide synthetases are modular proteins that operate in an assembly line fashion to bind, modify, and link amino acids. In the E. coli enterobactin NRPS system, the EntE adenylation domain catalyzes the transfer of a molecule of 2,3-dihydroxybenzoic acid to the pantetheine cofactor of EntB. We present here the crystal structure of the EntB protein that contains an N-terminal isochorismate lyase domain that functions in the synthesis of 2,3-dihydroxybenzoate and a C-terminal carrier protein domain. Functional analysis showed that the EntB-EntE interaction was surprisingly tolerant of a number of point mutations on the surface of EntB and EntE. Mutational studies on EntE support our previous hypothesis that members of the adenylate-forming family of enzymes adopt two distinct conformations to catalyze the two-step reactions.

References

Feb 1, 1996·Protein Science : a Publication of the Protein Society·E G Hutchinson, J M Thornton
Nov 1, 1996·Chemistry & Biology·R H LambalotC T Walsh
Aug 17, 1999·Current Opinion in Biotechnology·H D Mootz, M A Marahiel
Aug 30, 2001·Structure·G Y XuR Powers
Sep 22, 2001·Acta Crystallographica. Section D, Biological Crystallography·R J Read
Feb 5, 2002·The Journal of Biological Chemistry·Hing C WongJie Zheng
Feb 20, 2002·Chemical Reviews·Mohamed A. MarahielHenning D. Mootz
Sep 11, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jurgen J MayMilton T Stubbs
Mar 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Kenneth N RaymondSanggoo S Kim
Feb 11, 2004·Biochemistry·Gerwald Jogl, Liang Tong
Mar 3, 2004·Acta Crystallographica. Section D, Biological Crystallography·Laurent C StoroniRandy J Read
May 18, 2004·The Journal of Biological Chemistry·Yuko HisanagaMasashi Miyano
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Aug 31, 2004·Acta Crystallographica. Section D, Biological Crystallography·Xiayang Qiu, Cheryl A Janson
Dec 8, 2004·Current Opinion in Structural Biology·Gregory L Challis, James H Naismith
Feb 11, 2005·Chemical Reviews·Stephan A Sieber, Mohamed A Marahiel
May 13, 2005·Organic & Biomolecular Chemistry·Richard J PayneChris Abell
Jul 27, 2005·Biochemical Society Transactions·O KerbarhC Abell
Oct 4, 2005·Protein Science : a Publication of the Protein Society·Jonathan CaruthersEthan A Merritt
Jan 13, 2006·Nature Chemical Biology·Julian A FerrerasLuis E N Quadri

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Citations

Feb 22, 2012·Journal of Structural and Functional Genomics·Anna M GoralWladek Minor
Apr 3, 2010·Biochemistry·Alison L SikoraJohn S Blanchard
Oct 27, 2009·Biochemistry·Alison L SikoraJohn S Blanchard
Aug 26, 2009·Journal of the American Chemical Society·Dominique P FruehGerhard Wagner
Jul 29, 2009·Natural Product Reports·Alexander Koglin, Christopher T Walsh
Jul 29, 2010·Proceedings of the National Academy of Sciences of the United States of America·Marko MocibobIvana Weygand-Durasevic
Sep 7, 2007·Microbiology and Molecular Biology Reviews : MMBR·Marcus Miethke, Mohamed A Marahiel
Jul 4, 2007·Proceedings of the National Academy of Sciences of the United States of America·Zhe ZhouChristopher T Walsh
Feb 3, 2011·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Manuela Di LorenzoJorge H Crosa
Oct 17, 2015·Bioinformatics·Michael KnudsenChristian N S Pedersen
May 11, 2010·Analytical Biochemistry·Daniel J Wilson, Courtney C Aldrich
Sep 17, 2013·Journal of Molecular Biology·Puneet JunejaJennifer N Andexer
Sep 11, 2007·Biochemical and Biophysical Research Communications·Lianghui JiaHuarong Tan
Sep 26, 2007·Protein Science : a Publication of the Protein Society·Viktor Y AlekseyevChaitan Khosla
Oct 11, 2014·Biochemical and Biophysical Research Communications·Sofia KhalilPeter D Pawelek
Jul 30, 2016·Biochemical and Biophysical Research Communications·Paknoosh Pakarian, Peter D Pawelek
Jun 9, 2017·Metallomics : Integrated Biometal Science·Zachary L ReitzAlison Butler
Jun 26, 2017·Biochemical and Biophysical Research Communications·Jiansen DuQingjun Ma
Mar 13, 2018·Environmental Microbiology·Serina L RobinsonLawrence P Wackett
Jun 24, 2017·Natural Product Reports·Andrew M Gulick
Aug 7, 2012·Chembiochem : a European Journal of Chemical Biology·Viljemka Bučević-PopovićMaja Pavela-Vrančič

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