PMID: 9657722Jul 10, 1998Paper

Structure of the Escherichia coli RNA polymerase alpha subunit amino-terminal domain

Science
G Zhang, Seth A Darst

Abstract

The 2.5 angstrom resolution x-ray crystal structure of the Escherichia coli RNA polymerase (RNAP) alpha subunit amino-terminal domain (alphaNTD), which is necessary and sufficient to dimerize and assemble the other RNAP subunits into a transcriptionally active enzyme and contains all of the sequence elements conserved among eukaryotic alpha homologs, has been determined. The alphaNTD monomer comprises two distinct, flexibly linked domains, only one of which participates in the dimer interface. In the alphaNTD dimer, a pair of helices from one monomer interact with the cognate helices of the other to form an extensive hydrophobic core. All of the determinants for interactions with the other RNAP subunits lie on one face of the alphaNTD dimer. Sequence alignments, combined with secondary-structure predictions, support proposals that a heterodimer of the eukaryotic RNAP subunits related to Saccharomyces cerevisiae Rpb3 and Rpb11 plays the role of the alphaNTD dimer in prokaryotic RNAP.

References

Sep 5, 1991·Journal of Molecular Biology·R S HaywardA Ishihama
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Apr 1, 1995·Current Opinion in Genetics & Development·R H Ebright, S Busby
Sep 1, 1993·Microbiological Reviews·J Archambault, J D Friesen
Jul 20, 1993·Journal of Molecular Biology·B Rost, C Sander
May 1, 1993·Journal of Bacteriology·A Ishihama
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·D LaloP Thuriaux
Mar 1, 1996·The Journal of Biological Chemistry·T UlmasovT J Guilfoyle
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·T HeydukR H Ebright
Aug 1, 1997·Journal of Molecular Biology·Y WangS A Darst

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Citations

Mar 8, 2011·Cellular and Molecular Life Sciences : CMLS·Shigeharu Sato
Jul 19, 2012·World Journal of Microbiology & Biotechnology·Jeong Hyun KimBaek Rak Lee
Apr 23, 2004·Current Opinion in Structural Biology·Catherine L LawsonRichard H Ebright
Apr 12, 2001·Current Opinion in Structural Biology·S A Darst
Feb 13, 2003·Current Opinion in Structural Biology·Katsuhiko S Murakami, Seth A Darst
Feb 13, 2002·Current Opinion in Structural Biology·Patrick Cramer
Apr 4, 2000·Current Opinion in Microbiology·K Severinov
Nov 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Brian P HudsonCatherine L Lawson
Dec 16, 2000·The EMBO Journal·R D FinnM van Heel
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·S A DarstG Zhang
Nov 24, 2011·BMC Molecular Biology·Damir MukhamedyarovKonstantin Kuznedelov
Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Seth A DarstWilly Wriggers
Feb 7, 2001·Proceedings of the National Academy of Sciences of the United States of America·L MinakhinK Severinov
May 21, 2014·ELife·David DegenRichard H Ebright
May 15, 2015·Biomolecules·Katsuhiko S Murakami
Nov 10, 2009·Journal of Molecular Biology·William J Lane, Seth A Darst
Dec 13, 2006·Journal of Molecular Biology·Nora H BarakatJohn J Love
Mar 19, 2011·Protein Science : a Publication of the Protein Society·Kelly-Anne TwistLars F Westblade
Jul 8, 2010·Acta Crystallographica. Section D, Biological Crystallography·Samuel Lara-GonzálezCatherine L Lawson
Apr 19, 2006·Journal of Molecular Biology·Konstantin KuznedelovKonstantin Severinov
Feb 27, 1999·Current Opinion in Structural Biology·J Jäger, J D Pata
Apr 29, 2015·Proteins·Hua ChengNick V Grishin
Jul 18, 2015·Nature Communications·Heidi Redden, Hal S Alper

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