Structure of the has operon promoter and the effect of mutations on the has promoter strength in Streptococcus equi subsp. zooepidemicus

Molecular Biotechnology
Ján KrahulecJán Turňa

Abstract

The purpose of this study is to determine the effect of the corresponding nucleotides from Streptococcus pyogenes on the has promoter strength in highly encapsulated strain S. equi subsp. zooepidemicus (SEZ) and detect an empowering mutations in SEZ. Eight different strains of SEZ carrying nucleotide mutations in the -73 to -38 region upstream of the has promoter were constructed. The significant activity decrease to 36-1% was observed after the introduction of mutations in the promoter region from -44 to -38 site. The exception was observed in mutation in -49 site when no significant decrease was observed. When nucleotides TTT were used in positions -73 the promoter became weaker, whereas no significant effect was observed after using nucleotides CCC (96%). Unfortunately, introduction of these mutations into chromosome SEZ has no empowering effect. Six strains, which carried nucleotide sequences of different lengths upstream from the transcription start of hasA promoter, were constructed to determine the minimum upstream region required for the maximum transcription efficiency of the has operon. No change of the activity of the has promoter constructs containing as few as 101 nucleotides upstream from the transcription start p...Continue Reading

References

Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M R WesselsT J DiCesare
Jul 1, 1990·Molecular Microbiology·D A Morrison, B Jaurin
Sep 1, 1989·Equine Veterinary Journal·P M McCue, W D Wilson
Apr 25, 1983·Nucleic Acids Research·D K Hawley, W R McClure
Aug 4, 1995·The Journal of Biological Chemistry·D L Crater, I van de Rijn
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·M R Wessels, M S Bronze
Nov 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J CarapetisG Hogg
Nov 1, 1996·The Journal of Clinical Investigation·H M SchragerM R Wessels
Dec 16, 2004·Applied Microbiology and Biotechnology·Barrie Fong ChongLars K Nielsen
Oct 20, 2005·Journal of Bioscience and Bioengineering·Takashi Yamada, Takeru Kawasaki
Jan 16, 2007·Applied Microbiology and Biotechnology·Ján Krahulec, Jana Krahulcová
Feb 12, 2008·Current Protocols in Molecular Biology·D D Moore

❮ Previous
Next ❯

Citations

Nov 28, 2012·Molecular Biotechnology·Marcela TlustáJana Jílková
Jan 1, 2020·Journal of Biotechnology·Zbyněk ČernýVladimír Velebný

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.