PMID: 9430589Feb 7, 1998Paper

Structure of the HIV-1 nucleocapsid protein bound to the SL3 psi-RNA recognition element

Science
R N De GuzmanM F Summers

Abstract

The three-dimensional structure of the human immunodeficiency virus-type 1 (HIV-1) nucleocapsid protein (NC) bound to the SL3 stem-loop recognition element of the genomic Psi RNA packaging signal has been determined by heteronuclear magnetic resonance spectroscopy. Tight binding (dissociation constant, approximately 100 nM) is mediated by specific interactions between the amino- and carboxyl-terminal CCHC-type zinc knuckles of the NC protein and the G7 and G9 nucleotide bases, respectively, of the G6-G7-A8-G9 RNA tetraloop. A8 packs against the amino-terminal knuckle and forms a hydrogen bond with conserved Arg32, and residues Lys3 to Arg10 of NC form a 310 helix that binds to the major groove of the RNA stem and also packs against the amino-terminal zinc knuckle. The structure provides insights into the mechanism of viral genome recognition, explains extensive amino acid conservation within NC, and serves as a basis for the development of inhibitors designed to interfere with genome encapsidation.

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Citations

May 20, 1999·Biopolymers·R N De GuzmanM F Summers
May 20, 1999·Biopolymers·D J Patel
Jan 4, 2003·Chembiochem : a European Journal of Chemical Biology·Irene Gómez-PintoCarlos González
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Jul 29, 2006·Journal of the American Society for Mass Spectrometry·Kevin B TurnerDaniele Fabris
Mar 9, 2004·Journal of Molecular Biology·Victoria D'SouzaMichael F Summers
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Aug 3, 2005·Nature Reviews. Microbiology·Victoria D'Souza, Michael F Summers

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