Structure of the human APO-1 gene

European Journal of Immunology
I BehrmannP H Krammer

Abstract

APO-1/Fas (CD95) is a type 1 transmembrane protein that belongs to the tumor necrosis factor/nerve growth factor receptor family characterized by cysteine-rich extracellular domains. Cross-linking of APO-1 mediates apoptosis in a variety of cells. In the present study we report the isolation and characterization of the human APO-1 gene spanning approximately 25 kb of human chromosome 10. The gene consists of nine exons (25 bp to > 1.44 kb) separated by eight introns (152 bp to approximately 12 kb). The boundaries of exon 2 to 5 encoding the extracellular region do not match the boundaries of the three APO-1 protein subdomains. Exon structure and functional protein domains correspond for exon 6 encoding the transmembrane region and for exon 9 encoding the "death domain". By a polymerase chain reaction-based approach we localized major transcriptional start sites in human spleen cells 77 and 73 nucleotides upstream of the translation initiation codon of the human APO-1 gene. Minor initiation sites were found at positions -128, -111, -91, and -74. The 5' flanking sequence of the human APO-1 gene is GC rich, contains a high number of CpG dinucleotides and lacks a consensus TATA box. Consensus binding sites for the transcription fac...Continue Reading

References

Nov 1, 1992·Genomics·J InazawaS Nagata
Jan 11, 1992·Nucleic Acids Research·S Faisst, S Meyer
Aug 1, 1988·Molecular and Cellular Biology·A SehgalM Chao
Jul 1, 1994·Human Molecular Genetics·E Denamur, F F Chehab
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M AdachiS Nagata
Mar 15, 1993·Biochemical and Biophysical Research Communications·S KobayashiT Uede
Aug 1, 1993·The Journal of Experimental Medicine·J WuJ D Mountz
Jan 1, 1994·Annual Review of Immunology·P A Baeuerle, T Henkel
Jul 8, 1994·Science·A StoltzfusW F Doolittle
Apr 1, 1994·Current Opinion in Cell Biology·K Shuai
Sep 10, 1993·Cell·L A TartagliaD V Goeddel
Sep 1, 1993·Current Biology : CB·J F Bazan

❮ Previous
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Citations

Feb 14, 1998·Journal of Cellular Physiology·A FineN L Anderson
Aug 24, 2001·International Journal of Cancer. Journal International Du Cancer·A Aguilar-LemarroyF Rösl
Oct 1, 1996·Molecular and Cellular Biochemistry·H J Brömme, J Holtz
Jan 1, 1996·International Journal of Clinical & Laboratory Research·H J Gruss
Jun 12, 2013·Rheumatology International·Seyfi YıldırMehmet Emin Erdal
Aug 12, 2009·Journal of Hematopathology·Irina BonzheimThomas Rüdiger
Sep 27, 2003·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Li-E WangQingyi Wei
Mar 26, 2003·Seminars in Cancer Biology·Klaus Michael DebatinSimone Fulda
Aug 29, 1998·British Journal of Haematology·C BeltingerK M Debatin
Dec 8, 1998·The Journal of Experimental Medicine·M MüllerP H Krammer
May 1, 1996·DNA and Cell Biology·J YooC W Beattie
Mar 10, 1998·Thyroid : Official Journal of the American Thyroid Association·Y ShimaokaN Amino
Oct 28, 2003·Transplantation·Joost A B Van Der HoevenRutger J Ploeg
Nov 19, 2013·Current Opinion in Pediatrics·João Bosco Oliveira
Dec 31, 2009·Genes & Development·José M MorachisBeverly M Emerson
Mar 10, 1995·Science·S Nagata, P Golstein
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·M A GarcíaM Esteban
Mar 14, 1998·Molecular Pathology : MP·C P BeltingerK M Debatin
Nov 13, 2013·Pathology Oncology Research : POR·Behnaz ValibeigiFatemeh M Kamazani
Oct 31, 1996·Experientia·A SamaliT G Cotter
Nov 2, 2011·Cellular and Molecular Life Sciences : CMLS·Sébastien TauzinPatrick Legembre
Jul 19, 2013·Digestive Diseases and Sciences·Yueqiu QinSien Song
Sep 17, 2002·The Journal of Investigative Dermatology·Jean L UrquhartDavid A Norris
Jul 22, 2004·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·T MizunoH Tsujimoto
Apr 14, 2006·British Journal of Haematology·Austen WorthH Bobby Gaspar
Sep 14, 2013·American Journal of Reproductive Immunology : AJRI·Kentaro KaiHisashi Narahara
Apr 3, 2014·The Journal of Obstetrics and Gynaecology Research·Saeedeh SalimiMinoo Yaghmaei
Nov 21, 2015·Hepatitis Monthly·Asadollah MohammadiLida Jarahi
Jul 21, 2009·Biochemical and Biophysical Research Communications·Tobias SchillingMartina Müller
Nov 14, 2015·Journal of Clinical Immunology·María Gabriela Simesen de BielkeSilvia Danielian
Jan 13, 2006·The Journal of Urology·Hugh F O'KaneKate E Williamson

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