PMID: 9545595Apr 18, 1998Paper

Structure of the human Nkx2.1 gene

Biochimica Et Biophysica Acta
H HamdanP Minoo

Abstract

NKX2.1 is a member of the NK2 family of homeodomain-containing transcriptional factors which binds to and activates the promoters of thyroid and pulmonary epithelial genes. We have cloned and sequenced twelve human lung NKx2.1 cDNAs. To elucidate the origin of Nkx2.1 transcripts, we also cloned and sequenced a 12 kb human Nkx2.1 genomic clone. Alignment of cDNA sequences with the genomic clone showed that contrary to previous reports, the human Nkx2.1 gene is organized into three exons and two introns. The newly discovered exon I contains an ATG codon that falls in frame with the previously identified Nkx2.1 initiator ATG codon on one of the cDNAs, designated 5E. Northern blot analysis shows that an mRNA of approximately 2.5 kb in size, homologous to 5E, is expressed in both lung and thyroid. The deduced amino acid sequence of the longest open reading frame on 5E is identical to NKX2.1 with the exception of a 30 amino acid N-terminal extension. Coupled in vitro transcription/translation of the 5E cDNA confirms that the open reading frame is translated into a contiguous polypeptide of 44 kDa. Analysis of Nkx2.1 genomic DNA fragments suggest that at least two independent regions, one within the first intron and the other 5' of th...Continue Reading

References

Nov 1, 1991·Reviews of Infectious Diseases·M D LibmanJ Mendelson
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·Y Kim, M Nirenberg
Jan 22, 1982·Nucleic Acids Research·S M Mount
Apr 7, 1995·The Journal of Biological Chemistry·K IkedaJ A Whitsett
Jan 1, 1994·Annual Review of Physiology·P Minoo, R J King
Aug 11, 1994·Nucleic Acids Research·G DamanteR Di Lauro
Nov 15, 1994·Biochemical and Biophysical Research Communications·T EndoT Onaya
Jan 26, 1996·The Journal of Biological Chemistry·M ZanniniR Di Lauro
Dec 1, 1995·Developmental Biology·P MinooR deLemos
Jul 3, 2013·Investigative Ophthalmology & Visual Science·Shizuka KohKohji Nishida

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Citations

Dec 18, 2007·Annals of Human Genetics·M-M Garcia-BarcelóP K Tam
Jul 26, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Aparajita DasVijay Boggaram
Feb 21, 2002·The Journal of Clinical Investigation·Joachim PohlenzSamuel Refetoff
Feb 21, 2002·The Journal of Clinical Investigation·Heiko KrudeAnnette Grüters
Aug 24, 2010·Orphanet Journal of Rare Diseases·Annick ClementHarriet Corvol
Feb 7, 2001·Biochemical and Biophysical Research Communications·C LiP Minoo
Oct 13, 2005·Neurogenetics·Maria do Carmo CostaPatrícia Maciel
Oct 27, 2009·Journal of Pediatric Surgery·Thomas Y Y LeonMaria Mercedes Garcia-Barcelo
Aug 19, 2007·Movement Disorders : Official Journal of the Movement Disorder Society·Galit Kleiner-Fisman, Anthony E Lang
Jul 21, 2007·Annals of Human Genetics·Maria-Mercè Garcia-BarcelóPaul Kwong-hang Tam
Jul 28, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Carina M Rivolta, Héctor M Targovnik
Jul 23, 2015·Journal of Cell Communication and Signaling·Elger MartenChristiane E L Dammann
Dec 15, 2011·Journal of the Neurological Sciences·Mitsugu UematsuShigeo Kure
Jul 27, 2002·Developmental Biology·Changgong LiParviz Minoo
Oct 29, 2014·Nature Reviews. Endocrinology·Lara P FernándezPilar Santisteban
Mar 8, 2017·Scientific Reports·Shilpi MinochaCécile Lebrand
Nov 19, 2004·Human Molecular Genetics·Mercè Garcia-BarceloPaul K H Tam
Nov 6, 2012·Journal of Zhejiang University. Science. B·Li YangKe-jing Ying
Jan 23, 1999·The Journal of Biological Chemistry·J R Shaw-WhiteJ A Whitsett
Feb 27, 2001·The American Journal of Surgical Pathology·G PelosiG Viale
Apr 27, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·S W GlasserT R Korfhagen
Sep 27, 2019·European Respiratory Review : an Official Journal of the European Respiratory Society·Raphael BorieBruno Crestani
Jan 20, 2004·Cancer Research·Takuya FukazawaLaurie B Owen-Schaub

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