Structure of the O-Antigen and the Lipid A from the Lipopolysaccharide of Fusobacterium nucleatum ATCC 51191.

Chembiochem : a European Journal of Chemical Biology
Pilar Garcia-VelloCristina De Castro

Abstract

Fusobacterium nucleatum is a common member of the oral microbiota. However, this symbiont has been found to play an active role in disease development. As a Gram-negative bacterium, F. nucleatum has a protective outer membrane layer whose external leaflet is mainly composed of lipopolysaccharides (LPSs). LPSs play a crucial role in the interaction between bacteria and the host immune system. Here, we characterised the structure of the O-antigen and lipid A from F. nucleatum ssp. animalis ATCC 51191 by using a combination of GC-MS, MALDI and NMR techniques. The results revealed a novel repeat of the O-antigen structure of the LPS, [→4)-β-d-GlcpNAcA-(1→4)-β-d-GlcpNAc3NAlaA-(1→3)-α-d-FucpNAc4NR-(1→], (R=acetylated 60 %), and a bis-phosphorylated hexa-acylated lipid A moiety. Taken together these data showed that F. nucleatum ATCC 51191 has a distinct LPS which might differentially influence recognition by immune cells.

References

Feb 15, 1979·European Journal of Biochemistry·T Hofstad, G Fredriksen
Jan 1, 1982·Analytical Biochemistry·C M Tsai, C E Frasch
Jun 1, 1993·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·T HofstadK Sveen
Nov 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yasuyuki AsaiTomohiko Ogawa
Jun 3, 2009·DNA and Cell Biology·Maria AvilaOzlem Yilmaz
Jun 2, 2010·Nature Reviews. Microbiology·Paul E KolenbranderNicholas S Jakubovics
Dec 16, 2010·Journal of Chemical Information and Modeling·Philip V Toukach
Apr 27, 2012·Protein and Peptide Letters·Cristina De CastroAntonio Molinaro
Apr 1, 2014·Carbohydrate Research·K S Egorova, P V Toukach
Oct 30, 2014·Chemistry : a European Journal·Antonio MolinaroSonsoles Martín-Santamaría
Jan 13, 2015·Current Opinion in Microbiology·Yiping W Han
Aug 20, 2015·Nucleic Acids Research·Philip V Toukach, Ksenia S Egorova
Nov 26, 2015·International Reviews of Immunology·Anne GnauckMarlena C Kruger
Dec 15, 2015·Journal of Microbiological Methods·Gerald Larrouy-MaumusSerge Mostowy
Feb 16, 2017·Carbohydrate Research·Evgeny VinogradovAndrew D Cox
Jun 27, 2017·Carbohydrate Research·Evgeny VinogradovAndrew D Cox
May 14, 2018·Carbohydrate Research·Evgeny VinogradovAndrew D Cox
Dec 14, 2018·Nature Reviews. Microbiology·Caitlin A Brennan, Wendy S Garrett
Jan 17, 2019·FEMS Microbiology Reviews·Flaviana Di LorenzoAntonio Molinaro

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Citations

Aug 29, 2021·Pharmacology & Therapeutics·Pilar Garcia-VelloAntonio Molinaro
Oct 19, 2021·Frontiers in Immunology·Dimitra LamprinakiNathalie Juge

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