Structure of the O-glycans in GlyCAM-1, an endothelial-derived ligand for L-selectin.

The Journal of Biological Chemistry
S HemmerichS D Rosen

Abstract

L-selectin, the leukocyte selectin, mediates the carbohydrate-dependent attachment of circulating leukocytes to endothelium, preceding emigration into tissues. It functions in inflammatory leukocyte trafficking and in lymphocyte homing to lymph nodes. From previous work, the binding of L-selectin to endothelial-associated glycoprotein ligands, GlyCAM-1 and CD34, requires oligosaccharide sialylation, sulfation, and probably fucosylation. We have recently identified a major capping group in GlyCAM-1 as 6' sulfated sialyl Lewis x, a novel structure which potentially satisfies all of these requirements. In the present study, we define the complete structure of beta-eliminated chains of GlyCAM-1 using metabolic radiolabeling, plant lectin binding, and glycosidase digestions in conjunction with high pH anion-exchange chromatography. The majority of the O-glycans in GlyCAM-1 contain the T-antigen, i.e. Gal beta 1-->3GalNAc, which is incorporated into the core-2 structure, i.e. Gal beta 1-->3[GlcNAc beta 1-->6]GalNAc or larger core structures with additional GlcNAc residues. The structures of two O-glycans, based on core-2, were determined to be: [sequence: see text] The implications of these structures and more complex O-glycans for b...Continue Reading

References

Apr 15, 1992·European Journal of Biochemistry·F G Hanisch, J Peter-Katalinic
Nov 1, 1992·The Journal of Experimental Medicine·M BrusteinS R Watson
Jan 1, 1992·Annual Review of Immunology·L J Picker, E C Butcher
Feb 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A Varki
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M R Hardy, R R Townsend
Nov 1, 1984·Canadian Journal of Biochemistry and Cell Biology = Revue Canadienne De Biochimie Et Biologie Cellulaire·I BrockhausenH Schachter
Feb 1, 1994·Current Opinion in Immunology·R P McEver
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·A Varki
Oct 1, 1994·Current Opinion in Cell Biology·S D Rosen, C R Bertozzi
Feb 1, 1993·The Journal of Clinical Investigation·M P Bevilacqua, R M Nelson
Oct 15, 1993·Science·S BaumheterL A Lasky
Aug 1, 1993·Seminars in Immunology·S D Rosen
Sep 1, 1994·European Journal of Biochemistry·G M BrownI A Nieduszynski
Apr 26, 1994·Biochemistry·S Hemmerich, S D Rosen

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Citations

Nov 10, 1998·European Journal of Immunology·V V SwarteR E Mebius
Jan 3, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S Tsuboi, M Fukuda
Oct 9, 2002·Medicinal Research Reviews·Koichi Honke, Naoyuki Taniguchi
Jul 8, 2008·Glycoconjugate Journal·Estelle GarenauxYann Guérardel
Sep 27, 1996·Journal of Immunological Methods·M S Singer, S D Rosen
Oct 2, 2001·Carbohydrate Research·T YoshidaI Nakashima
Apr 25, 1997·Journal of Immunological Methods·C R BertozziS D Rosen
Aug 11, 2000·Transplantation Proceedings·P KwiatkowskiS Itescu
Dec 2, 1999·Biochimica Et Biophysica Acta·I Brockhausen
Dec 5, 2002·Current Opinion in Structural Biology·Reiji Kannagi
Oct 1, 1996·Current Opinion in Structural Biology·P R Crocker, T Feizi
Mar 23, 2002·Bioorganic & Medicinal Chemistry Letters·Christoph HohAndreas Liese
Sep 29, 1999·Trends in Biochemical Sciences·T Feizi, C Galustian
Sep 6, 2002·Bioorganic & Medicinal Chemistry·Jie XiaKhushi L Matta
Nov 13, 2004·Drug Discovery Today·Stefan HemmerichVirginia L Rath
Jul 17, 1998·Current Opinion in Chemical Biology·K J Yarema, C R Bertozzi
Jul 2, 2004·Organic Letters·Matthew R Pratt, Carolyn R Bertozzi
Mar 22, 2007·Nature Immunology·Ronen Alon, Steven Rosen
Oct 22, 2005·Nature Immunology·Rodger P McEver
Feb 14, 2009·Chemical Communications : Chem Comm·Jens DerneddeShahla Yekta
Jun 23, 1999·The Biochemical Journal·R MalhotraM I Bird
Jun 6, 2002·European Journal of Biochemistry·Anne YlönenNisse Kalkkinen
Feb 18, 2010·Proceedings of the National Academy of Sciences of the United States of America·Carolyn Bertozzi
Apr 12, 2012·The Journal of Biological Chemistry·Dan HuJun Hirabayashi
Aug 30, 2012·The Journal of Biological Chemistry·Chunsheng JinNiclas G Karlsson
Nov 5, 2003·The Journal of Experimental Medicine·Annemieke van ZanteSteven D Rosen
Nov 5, 2003·The Journal of Experimental Medicine·Christine M'RiniUlrich H von Andrian
Sep 16, 2000·Glycobiology·S Hemmerich, S D Rosen

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