PMID: 7019203Apr 1, 1981Paper

Structure of the peptidoglycan-associated lipoprotein (PAL) of the Proteus mirabilis outer membrane. I. Isolation and characterization of fatty acid-containing peptides from PAL

Journal of Biochemistry
T Mizuno

Abstract

In the outer membrane of P. mirabilis, a peptidoglycan-associated lipoprotein (PAL) of apparent molecular weight 18,000 is present as a major protein. A fatty acid-containing polypeptide (lipopolypeptide; LPP) was isolated by digestion of the purified PAL with trypsin in the presence of 0.05% SDS. It was composed of 31 amino acid residues, an unidentified compound[X], and ca. 3 fatty acid residues. A lipooligopeptide (LOP) was also isolated after further digestion of LPP with trypsin in the absence of SDS. LOP was composed of 4 amino acid residues (Asx, 2Ser, Lys), a compound[X], and ca. 3 fatty acid residues. The C-terminal amino acids of LPP and LOP were determined as arginine and lysine, respectively. On the other hand, the N-terminus of PAL, LPP, or LOP could not be identified by conventional N-terminal analysis of PAL, LPP, or LOP could not be identified by conventional N-terminal analysis, indicating that the N-terminus is probably masked. These results indicate that LPP and LOP are derived from the fatty-attached amino terminal region of PAL.

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.