Structure of the plasminogen kringle 4 binding calcium-free form of the C-type lectin-like domain of tetranectin

Biochemistry
Steen NielboH C Thøgersen

Abstract

Tetranectin is a homotrimeric protein containing a C-type lectin-like domain. This domain (TN3) can bind calcium, but in the absence of calcium, the domain binds a number of kringle-type protein ligands. Two of the calcium-coordinating residues are also critical for binding plasminogen kringle 4 (K4). The structure of the calcium free-form of TN3 (apoTN3) has been determined by NMR. Compared to the structure of the calcium-bound form of TN3 (holoTN3), the core region of secondary structural elements is conserved, while large displacements occur in the loops involved in calcium or K4 binding. A conserved proline, which was found to be in the cis conformation in holoTN3, is in apoTN3 predominantly in the trans conformation. Backbone dynamics indicate that, in apoTN3 especially, two of the three calcium-binding loops and two of the three K4-binding residues exhibit increased flexibility, whereas no such flexibility is observed in holoTN3. In the 20 best nuclear magnetic resonance structures of apoTN3, the residues critical for K4 binding span a large conformational space. Together with the relaxation data, this indicates that the K4-ligand-binding site in apoTN3 is not preformed.

References

Dec 1, 1994·The Journal of Cell Biology·U M WewerR Albrechtsen
Nov 1, 1995·Journal of Biomolecular NMR·F DelaglioA Bax
Feb 1, 1996·Journal of Molecular Graphics·R KoradiK Wüthrich
Jun 20, 1997·Journal of Molecular Biology·D E ZimmermanG T Montelione
Nov 21, 1997·Journal of Molecular Biology·P GüntertK Wüthrich
Jan 12, 2001·Physiological Reviews·E M Brown, R J MacLeod
Feb 5, 2003·Journal of Magnetic Resonance·Charles D SchwietersG Marius Clore
Apr 16, 2003·European Journal of Biochemistry·Uffe B WestergaardTorben E Petersen

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Citations

Dec 17, 2009·Glycoconjugate Journal·A-Rang ImYeong Shik Kim
Jul 30, 2014·Frontiers in Immunology·Jonas AretzChristoph Rademacher
Aug 26, 2016·Journal of the American Chemical Society·Jonas HanskeChristoph Rademacher
Dec 13, 2005·The FEBS Journal·Alex N Zelensky, Jill E Gready
Dec 16, 2019·Current Opinion in Structural Biology·Bettina G Keller, Christoph Rademacher
May 15, 2021·The Journal of Biological Chemistry·Jan-O JoswigBettina G Keller
Oct 20, 2007·Journal of the American Chemical Society·Daniela C PopescuNico A J M Sommerdijk

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