Structure of the pore-forming transmembrane domain of a ligand-gated ion channel

The Journal of Biological Chemistry
N MéthotJ E Baenziger

Abstract

The structure of the pore-forming transmembrane domain of the nicotinic acetylcholine receptor from Torpedo has been investigated by infrared spectroscopy. Treatment of affinity-purified receptor with either Pronase or proteinase K digests the extramembranous domains (roughly 75% of the protein mass), leaving hydrophobic membrane-imbedded peptides 3-6 kDa in size that are resistant to peptide (1)H/(2)H exchange. Infrared spectra of the transmembrane domain preparations exhibit relatively sharp and symmetric amide I and amide II band contours centered near 1655 and 1545 cm(-)1, respectively, in both (1)H(2)O and (2)H(2)O. The amide I band is very similar to the amide I bands observed in the spectra of alpha-helical proteins, such as myoglobin and bacteriorhodopsin, that lack beta structure and exhibit much less beta-sheet character than is observed in proteins with as little as 20% beta sheet. Curve-fitting estimates 75-80% alpha-helical character, with the remaining peptides likely adopting extended and/or turn structures at the bilayer surface. Infrared dichroism spectra are consistent with transmembrane alpha-helices oriented perpendicular to the bilayer surface. The evidence strongly suggests that the transmembrane domain of...Continue Reading

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Citations

Mar 7, 2013·The Journal of Biological Chemistry·Jonathan M LabriolaJohn E Baenziger
Dec 2, 2004·Brain Research. Brain Research Reviews·F J Barrantes
Aug 28, 2004·Journal of Molecular Graphics & Modelling·J R Trudell, E Bertaccini
Apr 16, 2009·Biochimica Et Biophysica Acta·Wajid M H Sayeed, John E Baenziger
Sep 8, 2009·Biochimica Et Biophysica Acta·Jacques Fantini, Francisco J Barrantes
Jun 26, 2002·Protein Engineering·E Bertaccini, J R Trudell
Apr 24, 2003·Medicinal Research Reviews·M Novella Romanelli, Fulvio Gualtieri
Feb 12, 2002·Nature Reviews. Neuroscience·Arthur Karlin
Jul 20, 2002·Molecular Pharmacology·María José De RosaCecilia Bouzat
Mar 17, 2004·The Journal of Biological Chemistry·Michael Cascio
Dec 19, 2014·The Journal of Biological Chemistry·Casey L CarswellJohn E Baenziger
Jan 27, 2005·Journal of the American Chemical Society·Yechun XuHualiang Jiang
Nov 1, 2002·Biochimica Et Biophysica Acta·M Montal, S J Opella
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Jan 26, 2005·Journal of Chemical Information and Modeling·Edward J BertacciniJames R Trudell

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