Structure of the pseudouridine synthase RsuA from Haemophilus influenzae

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Allan MatteStephen K Burley

Abstract

The structure of the pseudouridine synthase RsuA from Haemophilus influenza, which catalyzes the conversion of uridine to pseudouridine at a single position within 16S ribosomal RNA, has been determined at 1.59 A resolution and compared with that of Escherichia coli RsuA. The H. influenza enzyme contains an N-terminal S4-like alpha3beta4 domain followed by a catalytic domain, as observed in the structure of E. coli RsuA. Whereas the individual domains of E. coli and H. influenza RsuA are structurally similar, their relative spatial disposition differs greatly between the two structures. The former displays an extended open conformation with no direct contacts between the domains, while the latter is in a closed conformation with a large interface between the two domains. Domain closure presents several basic and polar residues into a putative RNA-binding cleft. It is proposed that this relative repositioning of the S4 and catalytic domains is used to modulate the shape and size of the rRNA-binding site in RsuA and in other pseudouridine synthases possessing S4 domains.

References

Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·C R KissingerD B Fogel
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·D CorolloR Fenna
Mar 27, 1999·Journal of Molecular Evolution·L Aravind, E V Koonin
Feb 17, 2000·The EMBO Journal·B L StakerM A Saper
Oct 3, 2000·Nature·B T WimberlyV Ramakrishnan
Sep 22, 2001·Protein Science : a Publication of the Protein Society·X QiuR L Jarvest
Jan 25, 2002·Acta Crystallographica. Section D, Biological Crystallography·John Badger, Jörg Hendle
Mar 21, 2002·FEBS Letters·James Ofengand
Apr 16, 2002·Nature Structural Biology·J SivaramanAllan Matte
Jul 12, 2002·The EMBO Journal·Anna YaremchukStephen Cusack
Oct 24, 2002·Acta Crystallographica. Section D, Biological Crystallography·M D WinnP Patel
Oct 21, 2003·Proceedings of the National Academy of Sciences of the United States of America·Hu PanRobert M Stroud
Mar 3, 2004·Nucleic Acids Research·Kulwadee Phannachet, Raven H Huang

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Citations

Aug 2, 2007·Journal of Structural and Functional Genomics·Allan MatteMiroslaw Cygler
Feb 27, 2007·Journal of Molecular Biology·Hu PanJanet Finer-Moore
Nov 23, 2006·Chemistry & Biology·Tomoko Hamma, Adrian R Ferré-D'Amaré

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