Structure of the recombinant alphavirus Western equine encephalitis virus revealed by cryoelectron microscopy.

Journal of Virology
Michael B Sherman, Scott C Weaver

Abstract

Western equine encephalitis virus (WEEV; Togaviridae, Alphavirus) is an enveloped RNA virus that is typically transmitted to vertebrate hosts by infected mosquitoes. WEEV is an important cause of viral encephalitis in humans and horses in the Americas, and infection results in a range of disease, from mild flu-like illnesses to encephalitis, coma, and death. In addition to spreading via mosquito vectors, human WEEV infections can potentially occur directly via aerosol transmission. Due to its aerosol infectivity and virulence, WEEV is thus classified as a biological safety level 3 (BSL-3) agent. Because of its highly infectious nature and containment requirements, it has not been possible to investigate WEEV's structure or assembly mechanism using standard structural biology techniques. Thus, to image WEEV and other BSL-3 agents, we have constructed a first-of-its-kind BSL-3 cryoelectron microscopy (cryoEM) containment facility. cryoEM images of WEEV were used to determine the first three-dimensional structure of this important human pathogen. The overall organization of WEEV is similar to those of other alphaviruses, consistent with the high sequence similarity among alphavirus structural proteins. Surprisingly, the nucleocaps...Continue Reading

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Citations

Aug 22, 2012·Proceedings of the National Academy of Sciences of the United States of America·Farooq NasarScott C Weaver
Apr 20, 2012·Journal of Virology·Helen E WhiteElena V Orlova
Dec 21, 2012·Journal of Virology·Nikos VasilakisRobert B Tesh
Dec 23, 2011·Journal of Virology·N L ForresterW I Lipkin
Apr 16, 2011·PloS One·Pramila WalpitaLinfa Wang
Jan 25, 2013·Therapeutic Delivery·Hazem AliErik Rytting
Jan 20, 2016·Expert Reviews in Molecular Medicine·Mahmoud IranpourSaeid Ghavami
Jan 1, 2013·Journal of Structural Biology·Michael B ShermanScott C Weaver
Oct 4, 2011·Current Opinion in Virology·Konstantin A TsetsarkinScott C Weaver
Jul 1, 2015·Journal of Virological Methods·J Jordan Steel, Brian J Geiss
Feb 13, 2014·MAbs·Birgit HülsewehMichael Hust
Sep 30, 2017·Viruses·Lindsay LundbergKylene Kehn-Hall
May 17, 2019·Science Translational Medicine·Sung-Youl KoSrinivas S Rao
Sep 24, 2019·Antibodies·Jinny L LiuGeorge P Anderson
Apr 5, 2020·Proceedings of the National Academy of Sciences of the United States of America·Chun-Liang ChenMichael G Rossmann
Feb 23, 2021·Integrative Biology : Quantitative Biosciences From Nano to Macro·Tianyi Jin, John Yin
Apr 4, 2021·Pharmaceuticals·Gill DiamondAnnelise E Barron
Aug 3, 2021·Advanced Materials·Chang-Heum JoSeung-Taek Myung
Aug 29, 2021·Pathogens·Margarita V Rangel, Kenneth A Stapleford

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