Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor.

Nature
Jun LanXinquan Wang

Abstract

A new and highly pathogenic coronavirus (severe acute respiratory syndrome coronavirus-2, SARS-CoV-2) caused an outbreak in Wuhan city, Hubei province, China, starting from December 2019 that quickly spread nationwide and to other countries around the world1-3. Here, to better understand the initial step of infection at an atomic level, we determined the crystal structure of the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 bound to the cell receptor ACE2. The overall ACE2-binding mode of the SARS-CoV-2 RBD is nearly identical to that of the SARS-CoV RBD, which also uses ACE2 as the cell receptor4. Structural analysis identified residues in the SARS-CoV-2 RBD that are essential for ACE2 binding, the majority of which either are highly conserved or share similar side chain properties with those in the SARS-CoV RBD. Such similarity in structure and sequence strongly indicate convergent evolution between the SARS-CoV-2 and SARS-CoV RBDs for improved binding to ACE2, although SARS-CoV-2 does not cluster within SARS and SARS-related coronaviruses1-3,5. The epitopes of two SARS-CoV antibodies that target the RBD are also analysed for binding to the SARS-CoV-2 RBD, providing insights into the future identification o...Continue Reading

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Citations

Apr 1, 2020·Nature·Jian ShangFang Li
May 5, 2020·Microbial Biotechnology·Harald Brüssow
Apr 2, 2020·ELife·Yinon M Bar-OnRon Milo
May 23, 2020·PLoS Pathogens·Daolin TangRui Kang
May 10, 2020·Science·R Kiplin GuyRebecca E Dutch
May 20, 2020·Journal of Clinical Medicine·Umesh KalathiyaJavier Antonio Alfaro
May 26, 2020·Journal of Biomolecular Structure & Dynamics·Ganesh Kumar VeeramachaneniJayakumar Singh Bondili
Jun 13, 2020·European Heart Journal. Cardiovascular Pharmacotherapy·Sophie LiabeufValérie Gras-Champel
May 8, 2020·Journal of Clinical Pathology·Tahir S Pillay
May 21, 2020·Allergy·Sergio Bonini, Giuseppe Maltese
Jun 4, 2020·Journal of Medical Virology·Rahsan Ilikci Sagkan, Dilara Fatma Akin-Bali
May 3, 2020·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Keshava RajagopalMarvin J Slepian
Jul 4, 2020·The World Journal of Biological Psychiatry : the Official Journal of the World Federation of Societies of Biological Psychiatry·Ulrike Schmidt, Theo Rein
Apr 16, 2020·Circulation·Peter P LiuHongliang Li
Jun 20, 2020·Dermatologic Therapy·Suruchi GargRitesh Agarwal
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Jul 15, 2020·Angewandte Chemie·Tingting YeBruno G De Geest

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Methods Mentioned

BETA
X-ray
gel filtration

Software Mentioned

CCP4 suite
wARP
COOT
PHENIX
ARP
Biacore Evaluation
PHASER

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