Structure of the Sir3 protein bromo adjacent homology (BAH) domain from S. cerevisiae at 1.95 A resolution.

Protein Science : a Publication of the Protein Society
Zhonggang HouJames L Keck

Abstract

Sir3p is a silent-information-regulator (SIR) protein required for the assembly of a transcriptionally "silent" chromatin structure at telomeres and the cryptic HM mating-type loci in Saccharomyces cerevisiae. Sir3p contains a putative "bromo adjacent homology" (BAH) domain at its N terminus that shares strong sequence similarity with the BAH domain of a subunit of the origin recognition complex (ORC), Orc1p. The Orc1p-BAH domain forms a well-defined complex with the ORC interaction region (OIR) of another Sir protein, Sir1p, which targets formation of silent chromatin to the HM-loci. Interestingly, despite sequence similarity of the Sir3p and Orc1p BAH domains and Sir3p's established importance in silencing, Sir3p does not bind the Sir1p-OIR. Here we report the 1.95 A resolution crystal structure of the Sir3p-BAH domain. The structure reveals two key features that can account for Sir3p-BAH domain's inability to interact with Sir1p. First, several Orc1p-BAH domain residues known to directly contact Sir1p are altered in the Sir3p-BAH domain. Second, a critical OIR-binding pocket present on the surface of the Orc1p-BAH domain is "filled" in the Sir3p-BAH domain structure, potentially making it inaccessible to Sir1p. These finding...Continue Reading

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Jun 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Zhonggang HouJames L Keck
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Citations

Aug 13, 2013·Nature Structural & Molecular Biology·Dongxue YangRui-Ming Xu
Aug 13, 2013·Nature Structural & Molecular Biology·Nadia ArnaudoFabrizio Martino
Mar 1, 2013·Journal of the Royal Society, Interface·Anna A KalashnikovaJeffrey C Hansen
Jan 19, 2010·Genes & Development·Anne Norris, Jef D Boeke
Sep 17, 2008·Molecular and Cellular Biology·Johannes R BuchbergerDanesh Moazed
Nov 28, 2012·Critical Reviews in Biochemistry and Molecular Biology·Na Yang, Rui-Ming Xu
Aug 16, 2016·Genetics·Marc R Gartenberg, Jeffrey S Smith
Apr 24, 2012·Biochimica Et Biophysica Acta·John J WyrickWilliam B Davis

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