Structure of the staphylococcal enterotoxin B vaccine candidate S19 showing eliminated superantigen activity

Acta Crystallographica. Section F, Structural Biology Communications
Woo Hyeon JeongSeong Tae Jeong

Abstract

Four mutations (N23A, Y90A, R110A and F177A) were introduced into S19, a vaccine candidate for staphylococcal enterotoxin B (SEB), resulting in a lower binding affinity towards the T-cell receptor beta chain (TCB) and reducing its superantigen activity. The structure of S19 was solved and was superposed on the native or complex structure of SEB. In the superposition model, mutations that were introduced seemed to reduce the number of hydrogen bonds at the SEB-TCB interface. S19 also displayed an unexpected structural change around the flexible-loop region owing to the Y90A mutation. This local structural change provided evidence that the mutated form of S19 could have a lower affinity for major histocompatibility complex (MHC) class II than wild-type SEB.

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Methods Mentioned

BETA
size-exclusion chromatography
PISA

Software Mentioned

PISA
HKL
PHENIX
ZDOCK

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