Structure of the Thermus thermophilus putative periplasmic glutamate/glutamine-binding protein

Acta Crystallographica. Section D, Biological Crystallography
Hitomi TakahashiTahir H Tahirov

Abstract

As part of a structural genomics project, the crystal structure of a 314-amino-acid protein encoded by Thermus thermophilus HB8 gene TT1099 was solved to 1.75 A using the multiple-wavelength anomalous dispersion (MAD) method and a selenomethionine-incorporated protein. The native protein structure was solved to 1.5 A using the molecular-replacement method. Both structures revealed a bound ligand, L-glutamate or L-glutamine, and a fold related to the periplasmic substrate-binding proteins (PSBP). Further comparative structural analysis with other PSBP-fold proteins revealed the conservation of the predicted membrane permease binding surface area and indicated that the T. thermophilus HB8 molecule is most likely to be an L-glutamate and/or an L-glutamine-binding protein related to the cluster 3 periplasmic receptors. However, the geometry of ligand binding is unique to the T. thermophilus HB8 molecule.

Citations

Mar 2, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Tomoe KitaoTahir H Tahirov
Mar 3, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Eiji InagakiTahir H Tahirov
Nov 2, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Ji Yong KangSe Won Suh
Oct 24, 2009·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Hyung Ho LeeSe Won Suh
Sep 10, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Thomas E EdwardsLance J Stewart
May 6, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·T Wubben, A D Mesecar
Sep 25, 2008·BMC Microbiology·Susana FragoMilagros Medina
Dec 7, 2013·Journal of Amino Acids·Janneth Gonzalez, George E Barreto
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Jun 30, 2010·FEMS Microbiology Reviews·Christopher MulliganGavin H Thomas
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Oct 22, 2019·Journal of Biomolecular Structure & Dynamics·Silvia CaprariFabio Polticelli
Feb 27, 2018·Frontiers in Cellular and Infection Microbiology·Leonardo T RosaDavid J Kelly

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