Structure of the UGAGAU hexaloop that braces Bacillus RNase P for action

Nature Structural Biology
T C LeeperSteven R Van Doren

Abstract

Long-range interactions involving the P5.1 hairpin of Bacillus RNase P RNA are thought to form a structural truss to support RNA folding and activity. We determined the structure of this element by NMR and refined the structure using residual dipolar couplings from a sample weakly oriented in a dilute liquid crystalline mixture of polyethylene glycol and hexanol. Dipolar coupling refinement improved the global precision of the structure from 1.5 to 1.2 A (to the mean), revised the bend angle between segments of the P5.1 stem and corroborated the structure of the loop region. The UGAGAU hexaloop of P5.1 contains two stacks of bases on opposite sides of the loop, distinguishing it from GNRA tetraloops. The unusual conformation of the juxtaposed uracil residues within the hexaloop may explain their requirement in transactivation assays.

Citations

Nov 8, 2002·Journal of Molecular Biology·Dominique FourmySatoko Yoshizawa
Jun 26, 2003·Journal of Molecular Biology·X-W FangT Pan
Feb 13, 2003·Biochemistry·Dipali G SashitalSamuel E Butcher
May 9, 2003·Nucleic Acids Research·Thomas LeeperGabriele Varani
Jul 31, 2003·Nucleic Acids Research·David W Staple, Samuel E Butcher
Jun 10, 2003·Microbiology and Molecular Biology Reviews : MMBR·Ciarán Condon
Feb 24, 2006·RNA·John C StrigglesFrancis J Schmidt

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