PMID: 6159600May 10, 1980Paper

Structure of transfer RNA by carbon NMR: resolution of single carbon resonances from 13C-enriched, purified species

Nucleic Acids Research
P F Agris, P G Schmidt

Abstract

Methyl carbon-13 NMR spectra of purified tRNA species are presented for the first time. In addition, these spectra of tRNA species specific for phenylalanine, tyrosine, and cysteine exhibited the first resolution of single methyl carbon resonances. Carbon-13 enriched methyl groups of ribothymidine (T) and 7-methylguanosine (m7G) and the methylthio group of 2-methylthio-N6-(delta2-isopentenyl) adenosine (ms2i6A) were resolved. The T methyl signal of tRNAPhe shifted from 12.3 ppm at 45 degrees in the absence of added Mg2+ to 11.1 ppm at 30 degrees in the presence of 10mM MgCl2. The same change in conditions led to a 0.4 ppm shift of the m7G methyl signal in the opposite direction. The relative ease in obtainment of single carbon resonances of purified tRNA species, and display of the sensitivity of their chemical shifts to changes in local structure, are requisite criteria for 13C-NMR to be a useful technique in probing tRNA conformation and its changes during interaction with proteins and other nucleic acids.

References

Jan 1, 1979·Nucleic Acids Research·D H GaussM Sprinzl
Mar 3, 1976·Journal of the American Chemical Society·W D HamillS Dickman
Jul 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·R A Komoroski, A Allerhand

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