Structure, stability, and thermodynamics of lamellar DNA-lipid complexes

Biophysical Journal
Daniel HarriesA Ben-Shaul

Abstract

We develop a statistical thermodynamic model for the phase evolution of DNA-cationic lipid complexes in aqueous solution, as a function of the ratios of charged to neutral lipid and charged lipid to DNA. The complexes consist of parallel strands of DNA intercalated in the water layers of lamellar stacks of mixed lipid bilayers, as determined by recent synchrotron x-ray measurements. Elastic deformations of the DNA and the lipid bilayers are neglected, but DNA-induced spatial inhomogeneities in the bilayer charge densities are included. The relevant nonlinear Poisson-Boltzmann equation is solved numerically, including self-consistent treatment of the boundary conditions at the polarized membrane surfaces. For a wide range of lipid compositions, the phase evolution is characterized by three regions of lipid to DNA charge ratio, rho: 1) for low rho, the complexes coexist with excess DNA, and the DNA-DNA spacing in the complex, d, is constant; 2) for intermediate rho, including the isoelectric point rho = 1, all of the lipid and DNA in solution is incorporated into the complex, whose inter-DNA distance d increases linearly with rho; and 3) for high rho, the complexes coexist with excess liposomes (whose lipid composition is differe...Continue Reading

References

Jan 26, 1989·Nature·P L Felgner, G M Ringold
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P L FelgnerM Danielsen
May 4, 1995·Biochimica Et Biophysica Acta·J GustafssonM Almgren
May 26, 1995·Science·B Honig, A Nicholls
Jul 22, 1996·FEBS Letters·T S TarahovskyG R Ivanitsky
Aug 1, 1996·Biophysical Journal·S W HuiK Chan
Sep 1, 1996·Biophysical Journal·N Dan
Nov 25, 1997·Biophysical Journal·S May, A Ben-Shaul
Jul 28, 1999·Journal of Colloid and Interface Science·J Stankovich, S L Carnie

❮ Previous
Next ❯

Citations

Jun 20, 2012·European Biophysics Journal : EBJ·Giulio Caracciolo, Heinz Amenitsch
Apr 1, 2008·Journal of Biological Physics·N S ChiaramoniS del V Alonso
Jul 4, 2012·Journal of Biosciences·Bidisha NandyPrabal K Maiti
May 16, 2000·Chemistry and Physics of Lipids·M I Angelova, I Tsoneva
Aug 10, 2001·Current Opinion in Structural Biology·C R Safinya
Oct 29, 2002·Bioelectrochemistry·V V Kuvichkin
Aug 11, 2010·The Journal of Physical Chemistry. B·Yinglong MiaoPeter J Ortoleva
Mar 1, 2012·The Journal of Physical Chemistry. B·Stephen D Pankavich, Peter J Ortoleva
Feb 22, 2014·Liquid Crystals·Cyrus R SafinyaYouli Li
Oct 2, 2002·Journal of Biomolecular Structure & Dynamics·R I ZhdanovG Bischoff
Aug 27, 2011·The European Physical Journal. E, Soft Matter·E R Teixeira da SilvaL Navailles
May 23, 2014·Journal of Photochemistry and Photobiology. B, Biology·Mudassir JabeenKushal Qanungo
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·I KoltoverC R Safinya
Aug 3, 2005·Proceedings of the National Academy of Sciences of the United States of America·Hongjun LiangGerard C L Wong
Feb 18, 2014·Advances in Colloid and Interface Science·Nily Dan, Dganit Danino
Apr 3, 2010·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Conchita Tros de IlarduyaNejat Düzgüneş
May 12, 2009·Chemistry and Physics of Lipids·Jean-François LabbéMichèle Auger
Mar 8, 2016·ACS Chemical Biology·Frederic GeinguenaudLaurence Motte
May 27, 2008·Biophysical Journal·Shelly TzlilAvinoam Ben-Shaul
Apr 8, 2009·IEEE Engineering in Medicine and Biology Magazine : the Quarterly Magazine of the Engineering in Medicine & Biology Society·Peter OrtolevaZeina Shreif
Nov 16, 2004·Biophysical Journal·F NataliA Congiu Castellano
Jan 30, 2007·Biophysical Journal·Oded Farago, Niels Grønbech-Jensen
Jan 1, 2005·Biophysical Journal·Emmanuel C MbamalaSylvio May
Apr 2, 2003·Biophysical Journal·Assaf ZemelAvinoam Ben-Shaul
Sep 26, 2013·Biochimica Et Biophysica Acta·Bruno F B SilvaCyrus R Safinya
Aug 9, 2011·Biochimica Et Biophysica Acta·Catarina MadeiraManuel Prieto
Aug 30, 2005·Biophysical Journal·Shelly Tzlil, Avinoam Ben-Shaul
Mar 3, 2004·Biophysical Journal·Elisabete GonçalvesTimothy D Heath
Apr 8, 2009·Biophysical Journal·Dganit DaninoDaniel Harries
Dec 4, 2003·Biophysical Journal·Edwin Pozharski, Robert C MacDonald
Feb 29, 2000·Biophysical Journal·M T KennedyR C MacDonald
Mar 29, 2000·Biophysical Journal·S MayA Ben-Shaul
Jun 25, 2002·Biophysical Journal·Edwin Pozharski, Robert C MacDonald
Jul 12, 2005·Biochimica Et Biophysica Acta·S SennatoP Malaspina
May 23, 2002·Biophysical Journal·Vadim CherezovMartin Caffrey

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.