Structure-toxicity relationship of phenolic analogs as anti-melanoma agents: an enzyme directed prodrug approach.

Chemico-biological Interactions
Nikhil M VadMajid Y Moridani

Abstract

The aim of this study was to identify a phenolic prodrug compound that is minimally metabolized by rat liver microsomes, but yet could form quinone reactive intermediates in melanoma cells as a result of its bioactivation by tyrosinase. In current work, we investigated 24 phenolic compounds for their metabolism by tyrosinase, rat liver microsomes and their toxicity towards murine B16-F0 and human SK-MEL-28 melanoma cells. A linear correlation was found between toxicities of phenolic analogs towards SK-MEL-28 and B16-F0 melanoma cells, suggesting similar mechanisms of toxicity in both cell lines. 4-HEB was identified as the lead compound. 4-HEB (IC(50) 48h, 75muM) showed selective toxicity towards five melanocytic melanoma cell lines SK-MEL-28, SK-MEL-5, MeWo, B16-F0 and B16-F10, which express functional tyrosinase, compared to four non-melanoma cells lines SW-620, Saos-2, PC3 and BJ cells and two amelanotic SK-MEL-24, C32 cells, which do not express functional tyrosinase. 4-HEB caused significant intracellular GSH depletion, ROS formation, and showed significantly less toxicity to tyrosinase specific shRNA transfected SK-MEL-28 cells. Our findings suggest that presence of a phenolic group in 4-HEB is critical for its selective ...Continue Reading

References

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Citations

Aug 6, 2010·Chemico-biological Interactions·Shashi K KuduguntiMajid Y Moridani
Oct 24, 2012·Journal of Biomedicine & Biotechnology·Ahmad Firdaus B LajisArbakariya B Ariff
Sep 6, 2020·Ecotoxicology and Environmental Safety·Nusiba Mohammed Modawe Alshik Edris, Yusran Sulaiman
Nov 9, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Dongdong LiJianbin Tang

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