PMID: 9923012Jan 29, 1999Paper

Structure/antigenicity relationship of cyclic and linear peptides mimicking the V3 loop of HIV2 envelope glycoprotein

Research in Virology
B Belhadj JradE Bahraoui

Abstract

We report the structure and antigenicity of the third variable region (V3) of the HIV2 envelope glycoprotein by the use of linear and cyclic peptides. To this end, a peptide mimicking this region was synthesized and purified, both as an iodoacetamidated linear peptide and a disulphide-bridged cyclic peptide. The cross-reactivity of three monoclonal antibodies (mAbs) produced against the envelope glycoprotein gp140 with the linear and cyclic peptides was tested with ELISA. The results showed that the cyclic peptide is a better ligand for the 3 mAbs 125-F, 125-J and 125-K. The avidity of the mAb/peptide interaction was further analysed by determining the concentration of linear or cyclic peptide leading to 50% inhibition of mAb-peptide complex formation (K0.5). The K0.5 value of mAb 125-F, which displayed the best reactivity with gp140, was estimated to be 5 times higher for the linear (K0.5 = 1.5 x 10(-6) M) than for the cyclic peptide (K0.5 = 3 x 10(-7) M). This indicates a higher affinity of mAb 125-F for the cyclic peptide. mAb 125-J, which exhibited a lower avidity for the gp140 compared to mAb 125-F, had a similar affinity for the cyclic and the linear peptides (K0.5 = 3 x 10(-7) M). mAb 125-K had the lowest reactivity with...Continue Reading

References

Apr 1, 1988·AIDS·R A WeissL Montagnier
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J F ZaguryF Laure
Apr 18, 1986·Science·B Merrifield
Apr 1, 1994·Science·J B GhiaraI A Wilson
Jan 1, 1993·Archives of Virology·C JoissonM H Van Regenmortel
Feb 3, 1995·The Journal of Biological Chemistry·P CatastiG Gupta
Dec 1, 1994·AIDS Research and Human Retroviruses·F TraincardS Benichou
Jun 10, 1996·AIDS Research and Human Retroviruses·J AlbertF Chiodi
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN

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