PMID: 12763860May 24, 2003Paper

Structure/function studies of the gamma subunit of the Na,K-ATPase

Annals of the New York Academy of Sciences
Rhoda BlosteinAthina Zouzoulas

Abstract

The Na,K-ATPase gamma subunit is present primarily in kidney as two splice variants, gammaa and gammab, which differ only at their extracellular N-termini. Two distinct effects of gamma are seen in biochemical Na,K-ATPase assays of mammalian (HeLa) cells transfected with gammaa or gammab, namely, (i) a decrease in K'(ATP) probably secondary to a shift in steady-state E(1) <--> E(2) poise in favor of E(1) and (ii) an increase in cytoplasmic K(+)/Na(+) antagonism seen as an increase in K'(Na) at high K(+) concentration. Mutagenesis experiments involving alterations in extramembranous regions of gamma indicate that different regions mediate the aforementioned distinct effects and that the effects appear to be long range. Studies of ouabain-sensitive fluxes with intact cells confirm the gamma effects seen with membranes and also suggest an additional effect (increase) in apparent affinity for extracellular K(+). Alteration in gamma function was also evidenced in the behavior of a G41 -->R mutation within the transmembrane domain of gamma. G41R is associated with autosomal dominant renal magnesium wasting. Our studies show that this mutation in the gammab variant retards trafficking of gamma, but not alphabeta pumps, to the cell sur...Continue Reading

References

Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·B AttaliH Garty
Jan 24, 1998·The Journal of Biological Chemistry·A G TherienR Blostein
Jan 5, 2002·Journal of Bioenergetics and Biomembranes·A G TherienR Blostein
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Feb 8, 2002·American Journal of Physiology. Renal Physiology·Elena ArystarkhovaKathleen J Sweadner
Apr 4, 2002·The Journal of Biological Chemistry·Helen X PuRhoda Blostein
Jul 3, 2002·The EMBO Journal·Pascal BéguinKäthi Geering
Sep 10, 2002·American Journal of Physiology. Renal Physiology·Haim GartySteven J D Karlish
May 24, 2003·Annals of the New York Academy of Sciences·Käthi GeeringGilles Crambert

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Citations

Jan 14, 2011·The Journal of Biological Chemistry·Neeraj Kumar MishraSteven J D Karlish
Aug 29, 2014·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Martin Konrad, Karl Peter Schlingmann
May 25, 2005·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Philip R BrauerDavid H Petzel
Jun 8, 2006·American Journal of Physiology. Renal Physiology·Kaarina Pihakaski-MaunsbachArvid B Maunsbach
Nov 24, 2017·Nephron·Luca ZanoliPasquale Fatuzzo
Sep 4, 2018·Physiological Reports·Haim MayanSteven J D Karlish
Jan 23, 2021·Animals : an Open Access Journal From MDPI·Qianqian SongGuohong Chen

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