Structures of apomyoglobin's various acid-destabilized forms

Biochemistry
R GilmanshinR H Callender

Abstract

The structures and the cold and hot melting thermodynamics of the acid- and salt-destabilized states of horse heart apomyoglobin (apoMb), including the E (extended) and various I forms, are studied using probes of tertiary structure (tryptophan fluorescence and FTIR spectroscopy) and secondary structure (far-UV CD and FTIR spectroscopy). These forms likely resemble early structures in the folding of the largely helical protein. Both the I and E forms retain the AGH core whereby the two ends of the protein are tied together with sufficient numbers of tertiary contacts, involving a number of hydrophobic residues, to show cooperative melting. The melting thermodynamics of E and I are distinctly different. E contains no other tertiary structure and probably little other secondary structure apart from the core. The more destabilized E form appears to contain "random" buried runs of polypeptide backbone which convert to alpha-helix in the I form(s). Most interestingly, E consists not of a single structure but is composed of a heterogeneous mixture of conformations, all showing corelike cooperative melting characteristics, and consisting presumably of varying contacts between the A portion of apomyoglobin and the G-H hairpin. These re...Continue Reading

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Citations

Sep 5, 2002·Mass Spectrometry Reviews·Igor A Kaltashov, Stephen J Eyles
Sep 13, 2002·Journal of Molecular Biology·Chiaki NishimuraPeter E Wright
Jun 7, 2007·Journal of the American Chemical Society·Michael A BryanRichard Mendelsohn
Oct 26, 2012·Journal of the American Chemical Society·Gurusamy BalakrishnanThomas G Spiro
May 21, 2004·Protein Science : a Publication of the Protein Society·Paola PicottiAngelo Fontana
Feb 26, 2013·Protein Science : a Publication of the Protein Society·Igor A KaltashovRinat R Abzalimov
Jun 20, 2006·Biochimica Et Biophysica Acta·Klaus V KilimannMichael G Gänzle
Mar 1, 2003·Biophysical Journal·Miriam GulottaR Brian Dyer
Aug 12, 2004·Chemical Reviews·H Jane Dyson, Peter E Wright
Jul 12, 2008·The Journal of Physical Chemistry. B·Mariangela Dametto, Alfredo E Cárdenas
Jul 21, 2006·The Journal of Physical Chemistry. B·Aleksandr V Mikhonin, Sanford A Asher

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