Structures of archaeal DNA segregation machinery reveal bacterial and eukaryotic linkages

Science
Maria A SchumacherDaniela Barillà

Abstract

Although recent studies have provided a wealth of information about archaeal biology, nothing is known about the molecular basis of DNA segregation in these organisms. Here, we unveil the machinery and assembly mechanism of the archaeal Sulfolobus pNOB8 partition system. This system uses three proteins: ParA; an atypical ParB adaptor; and a centromere-binding component, AspA. AspA utilizes a spreading mechanism to create a DNA superhelix onto which ParB assembles. This supercomplex links to the ParA motor, which contains a bacteria-like Walker motif. The C domain of ParB harbors structural similarity to CenpA, which dictates eukaryotic segregation. Thus, this archaeal system combines bacteria-like and eukarya-like components, which suggests the possible conservation of DNA segregation principles across the three domains of life.

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Citations

May 6, 2016·Scientific Reports·Laura Molina-GarcíaRafael Giraldo
Jun 28, 2016·Genome Biology and Evolution·Sriram G Garg, William F Martin
Jul 28, 2016·Trends in Microbiology·Daniela Barillà
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Nov 18, 2018·Molecular Systems Biology·Roxanne E DebaugnyJean-Yves Bouet
Oct 28, 2019·Science·Young-Min SohStephan Gruber
Jun 17, 2020·Open Biology·Adam S B Jalal, Tung B K Le
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May 16, 2017·Nature Reviews. Microbiology·Alexander WagnerSonja-Verena Albers
Jul 18, 2020·Molecular Cell·Baptiste GuilhasMarcelo Nollmann

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