Structures of the Erythrina corallodendron lectin and of its complexes with mono- and disaccharides

Journal of Molecular Biology
S Elgavish, B Shaanan

Abstract

The structures of the Erythrina corallodendron lectin (EcorL) and of its complexes with galactose, N-acetylgalactosamine, lactose and N-acetyllactosamine were determined at a resolution of 1.9 to 1.95 A. The final R-values of the five models are in the range 0.169 to 0.181. The unusual, non-canonical, dimer interface of EcorL is made of beta-strands from the two monomers, which face one another in a "hand-shake" mode. The galactose molecule in the primary binding site is bound in an identical way in all four complexes. Features of the electrostatic potential of the galactose molecule match those of the potential in the combining site, thus probably pointing to the contribution of the electrostatic energy to determining the orientation of the ligand. No conformational change occurs in the protein upon binding the ligand. Subtle variations in the binding mode of the second monosaccharide (glucose in the complex with lactose and N-acetylglucosamine in the complex with N-acetyllactosamine) were observed. The mobility of Gln219 is lower in the complexes with the disaccharides than in the complexes with the monosaccharides, indicating further recruitment of this residue to ligand binding through more extensive hydrogen bonding in the...Continue Reading

References

Jan 1, 1991·Proteins·E Rutenber, J D Robertus
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Oct 13, 1989·Science·N Sharon, H Lis
Dec 19, 1972·Biochemistry·K D Hardman, C F Ainsworth
Jan 1, 1968·Advances in Protein Chemistry·G N Ramachandran, V Sasisekharan
May 5, 1982·Journal of Molecular Biology·K D HardmanM J Freiser
Feb 1, 1995·Protein Engineering·A C WallaceJ M Thornton
Jan 1, 1995·Annual Review of Biophysics and Biomolecular Structure·J M Rini
Dec 15, 1993·Structure·M Levitt, B H Park
Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·D I LiaoO Herzberg
Jan 12, 1996·The Journal of Biological Chemistry·J H Naismith, R A Field
Jul 26, 1996·The Journal of Biological Chemistry·A SuroliaF P Schwarz
Aug 15, 1996·Structure·G J Kleywegt, A T Brünger
Jan 1, 1996·Annual Review of Biochemistry·W I Weis, K Drickamer
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·P D AdamsA T Brünger
Jan 20, 1998·Trends in Biochemical Sciences·S Elgavish, B Shaanan
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Jan 1, 1993·Acta Crystallographica. Section D, Biological Crystallography·V S Lamzin, K S Wilson

❮ Previous
Next ❯

Citations

Jun 26, 2001·Allergy·R C AalberseR van Ree
Mar 2, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Ruth MikeskaChristian Betzel
Mar 29, 2001·Protein Science : a Publication of the Protein Society·S Elgavish, B Shaanan
Mar 5, 2004·Proteins·Roberto D LinsPhilippe H Hünenberger
Dec 13, 2007·Journal of Computational Chemistry·Anthony D Hill, Peter J Reilly
Jul 10, 2008·Chemistry : a European Journal·Anna Bernardi, Pavel Cheshev
Aug 23, 2008·Chemistry : a European Journal·Emilio J CocineroJohn P Simons
Apr 5, 2012·Chemistry : a European Journal·Christopher J PaceJianmin Gao
May 26, 2004·Archives of Biochemistry and Biophysics·Eric BonneilPierre Thibault
Oct 6, 1999·Current Opinion in Structural Biology·J BouckaertR Loris
Feb 15, 2003·FEBS Letters·Alvaro BabinoPedro M Alzari
May 18, 2016·Proceedings of the National Academy of Sciences of the United States of America·Mark J G BakkersRaoul J de Groot
Nov 22, 2011·Acta Crystallographica. Section D, Biological Crystallography·Jürgen J MüllerUdo Heinemann
Aug 23, 2012·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S BlankE Spillner
Oct 14, 2003·Bioorganic & Medicinal Chemistry Letters·Daniela ArosioAnna Bernardi
Mar 24, 2005·The Journal of Biological Chemistry·James FlintHarry J Gilbert
Apr 29, 2005·Organic & Biomolecular Chemistry·Moira AmbrosiBenjamin G Davis
Jul 27, 2001·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·E Pidcock, G R Moore
Mar 3, 1999·Journal of Biomolecular Structure & Dynamics·M S VanLoockS C Harvey
Jan 12, 2002·The Journal of Biological Chemistry·Linda J OlsonJung-Ja P Kim
Jan 14, 2004·The Journal of Biological Chemistry·Christian Appenzeller-HerzogHans-Peter Hauri
Apr 1, 2011·Chemical Communications : Chem Comm·Francisco CorzanaJesús M Peregrina
May 13, 2018·Amino Acids·Oliviero Carugo
Jan 13, 2019·International Journal of Molecular Sciences·Annick BarrePierre Rougé

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.