PMID: 7541354Jun 15, 1995Paper

Structures of the N-linked oligosaccharides on human plasma vitronectin

European Journal of Biochemistry
H OgawaI Matsumoto

Abstract

The structures of N-linked oligosaccharides present on human plasma vitronectin were elucidated. Oligosaccharides were released from the vitronectin by N-glycosidase F digestion and tagged with 2-aminopyridine; the pyridylamino-oligosaccharides were then fractionated by anion-exchange and reverse-phase HPLC. Ten major pyridylamino-oligosaccharides were isolated. The linkages and locations of sialic acid residues were determined by desialylation with Salmonella sialidase in combination with acid. The asialo forms were then analyzed by two-dimensional sugar mapping, component sugar analysis and 400-MHz 1H-NMR spectroscopy. The major oligosaccharides of human vitronectin were of the diantennary N-acetyllactosamine type, with a lesser amount of the tri- and a small amount of the mono-antennary type, to which 1-3 mol sialic acid residues were linked, mostly through alpha 2-6 linkages, although alpha 2-3 linkages were also present. The possibility that several binding activities of vitronectin can be ascribed to its glycan moiety was discussed, based on the specific features of the N-linked oligosaccharides on human vitronectin revealed here.

References

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Feb 6, 2013·Apoptosis : an International Journal on Programmed Cell Death·Nadia MalagoliniFabio Dall'Olio
Sep 12, 2007·Journal of Molecular Histology·Yvonne D'souzaRichard Bonshek
Jun 4, 1998·Thrombosis Research·K T Preissner, D Seiffert
Aug 30, 2008·Chemistry : a European Journal·Katelijne GheysenJosé C Martins
Nov 12, 2015·Glycoconjugate Journal·Florent ClercManfred Wuhrer
Nov 11, 2009·Journal of Proteome Research·Hyoung-Joo LeeYoung-Ki Paik
Aug 9, 2016·Journal of Proteome Research·Suzanne MiyamotoGary S Leiserowitz
Jan 15, 2013·Journal of Proteome Research·Catarina GomesHugo Osório

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