Structures of transcription pre-initiation complex with TFIIH and Mediator.

Nature
S SchilbachP Cramer

Abstract

For the initiation of transcription, RNA polymerase II (Pol II) assembles with general transcription factors on promoter DNA to form the pre-initiation complex (PIC). Here we report cryo-electron microscopy structures of the Saccharomyces cerevisiae PIC and PIC-core Mediator complex at nominal resolutions of 4.7 Å and 5.8 Å, respectively. The structures reveal transcription factor IIH (TFIIH), and suggest how the core and kinase TFIIH modules function in the opening of promoter DNA and the phosphorylation of Pol II, respectively. The TFIIH core subunit Ssl2 (a homologue of human XPB) is positioned on downstream DNA by the 'E-bridge' helix in TFIIE, consistent with TFIIE-stimulated DNA opening. The TFIIH kinase module subunit Tfb3 (MAT1 in human) anchors the kinase Kin28 (CDK7), which is mobile in the PIC but preferentially located between the Mediator hook and shoulder in the PIC-core Mediator complex. Open spaces between the Mediator head and middle modules may allow access of the kinase to its substrate, the C-terminal domain of Pol II.

References

Feb 1, 1996·Journal of Molecular Graphics·W HumphreyK Schulten
Sep 1, 1996·Trends in Biochemical Sciences·R G Roeder
Jul 31, 1999·The Journal of Biological Chemistry·R J MorelandR C Conaway
Sep 28, 2000·Cell·P SchultzJ M Egly
Apr 12, 2002·Trends in Biochemical Sciences·Tobias DoerksPeer Bork
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
May 18, 2005·Trends in Biochemical Sciences·Roger D Kornberg
Jun 7, 2005·Nature Structural & Molecular Biology·Yin Chun LinJay D Gralla
Nov 3, 2005·The Journal of Biological Chemistry·Yuichiro Takagi, Roger D Kornberg
Jul 25, 2006·Journal of Structural Biology·Guang TangSteven J Ludtke
Jun 27, 2008·PLoS Biology·Stefanie C WolskiCaroline Kisker
Jan 10, 2009·Nature Protocols·Lorenza BordoliTorsten Schwede
Jan 28, 2009·Nature Structural & Molecular Biology·Denis E KainovJean-Marc Egly
Aug 25, 2009·Proteins·Srivatsan RamanDavid Baker
Nov 26, 2009·DNA Repair·M StefaniniD Orioli
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Meigang Gu, Charles M Rice
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
May 12, 2010·Current Opinion in Structural Biology·Margaret E Fairman-WilliamsEckhard Jankowsky
Feb 8, 2011·Journal of Structural Biology·Matthew L BakerTao Ju
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Brian J GibbonsRoger D Kornberg

❮ Previous
Next ❯

Citations

Dec 7, 2018·ELife·Kaiying Cheng, Dale B Wigley
Feb 15, 2019·ELife·Stephen M Hinshaw, Stephen C Harrison
Mar 13, 2019·ELife·Basil J GreberEva Nogales
Jun 9, 2018·Nature Reviews. Drug Discovery·Jean-Paul RenaudChristian Wiesmann
Jul 13, 2019·Nucleic Acids Research·Adrien GeorgesJulie Soutourina
Jul 10, 2019·Proceedings of the National Academy of Sciences of the United States of America·Tyler HeistMichael Levine
Jul 6, 2019·The Plant Journal : for Cell and Molecular Biology·Zhoubo HuCsaba Koncz
May 28, 2019·Genes & Development·Leighton Core, Karen Adelman
Apr 18, 2018·Protein Science : a Publication of the Protein Society·Jenna K Rimel, Dylan J Taatjes
Jan 23, 2020·International Journal of Molecular Sciences·Mario Zurita, Juan Manuel Murillo-Maldonado
Jan 4, 2019·Acta Crystallographica. Section F, Structural Biology Communications·C O S SorzanoJ M Carazo
May 14, 2020·Transcription·Danielle E LyonsMelanie Ott
Aug 30, 2019·Nature·Patrick Cramer
Aug 29, 2020·Proceedings of the National Academy of Sciences of the United States of America·Basil J GreberEva Nogales
May 10, 2018·FEMS Microbiology Reviews·Malcolm F White, Thorsten Allers
Feb 3, 2019·Biochemical Society Transactions·Kevin KrammDina Grohmann
Jun 30, 2019·Nature Communications·Goran KokicPatrick Cramer
Aug 22, 2018·Nature Structural & Molecular Biology·Marc BoehningMarkus Zweckstetter
May 22, 2019·Nature Structural & Molecular Biology·Chunli YanIvaylo Ivanov
Mar 5, 2019·Annual Review of Biochemistry·Merav BraitbardNir Kalisman
Aug 11, 2019·Nature Reviews. Genetics·Erin M WissinkJohn T Lis
Apr 3, 2020·Genes & Development·Allison C Schier, Dylan J Taatjes
Mar 7, 2020·Nature Communications·Michael Pilsl, Christoph Engel
Feb 9, 2019·Biochemical Society Transactions·Alexis VergerVincent Villeret
Aug 24, 2018·Nature·Seychelle M VosPatrick Cramer
May 2, 2020·Nature Communications·Yana van der WeegenMartijn S Luijsterburg
Apr 5, 2020·Nature Communications·Stefan PeissertCaroline Kisker
Oct 2, 2020·Nature Communications·Matthias K VorländerChristoph W Müller
Sep 8, 2018·Nature Structural & Molecular Biology·Hauke S HillenPatrick Cramer
Nov 7, 2019·Nature Structural & Molecular Biology·Yong WeiMaria Sunnerhagen
May 13, 2020·Inverse Problems·Amit MoscovichAmit Singer
Dec 24, 2018·Biochemical Society Transactions·Christoph Engl
Aug 22, 2020·Annual Review of Cell and Developmental Biology·Sara Osman, Patrick Cramer
Nov 20, 2020·Nucleic Acids Research·Masahiko OkudaYoshifumi Nishimura
Oct 24, 2020·Genome Research·Jacques SerizayJulie Ahringer
Nov 2, 2019·Cell·Patrick Cramer

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
ion
size exclusion chromatography
gel filtration
NMR
size-exclusion chromatography

Software Mentioned

MDFF
XLink Analyzer Plugin
pLink
WarpCraft
MotionCor2
EMAN2
xiNET
RELION
cMed
e2boxer

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.