Structures of two novel crystal forms of Naja naja naja phospholipase A2 lacking Ca2+ reveal trimeric packing

Journal of Molecular Biology
B W SegelkeE A Dennis

Abstract

Three crystal forms of Naja naja naja phospholipase A2 were discovered through random crystallization screening, including two heretofore uncharacterized forms. The crystallization conditions for both of these novel crystal forms are Ca(2+)-free whereas previously reported conditions include Ca2+. One of the new crystal forms has a cubic lattice in the space group P2(1)3 (a = b = c = 69.24 A), the other has an orthorhombic lattice in the space group P2(1)2(1)2(1) (a = 67.22 A, b = 73.48 A, c = 87.52 A) and a previously characterized crystal belong to the tetragonal space group P4(3)2(1)2 (a = b = 88.6 A, c = 107.4 A). The structure from the cubic crystal form has been determined to 1.8 A and refined to an R-factor of 17% while the structure from the orthorhombic form has been determined to 2.65 A and has been refined to an R-factor of 21%. The determination of the cubic structure extends the resolution to which structures of this molecule have been determined from 2.3 A to 1.8 A. The two newly determined structures, in combination with the previously determined structure, generate an informative structural ensemble from which structural changes due to Ca2+, which is required for catalysis, and the effect of crystal contacts on ...Continue Reading

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Citations

Oct 22, 2008·Cardiovascular Drugs and Therapy·John E Burke, Edward A Dennis
Mar 20, 2009·The Journal of Biological Chemistry·Wei XuJinsong Liu
Dec 23, 2004·Bioinformatics·Brian PierceZhiping Weng
Mar 2, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Garima SinghT P Singh
Feb 15, 2000·American Journal of Respiratory and Critical Care Medicine·E A Dennis
Jan 8, 2016·The Journal of Physical Chemistry. B·Zhong-Ru XieYinghao Wu
Jun 22, 2002·Toxicon : Official Journal of the International Society on Toxinology·Lichuan GuZhengjiong Lin
Jan 16, 2015·Journal of Lipid Research·Jere P SegrestSaravana P Thirumuruganandham
Jun 1, 2016·Journal, Genetic Engineering & Biotechnology·S T V RaghavammaGarikapati Devala Rao
Nov 21, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Carmen X Luzuriaga-QuichimboTrinidad Ruiz-Téllez
Oct 15, 2019·Journal of Biomolecular Structure & Dynamics·Shafi MahmudN Absar
May 2, 2013·Acta Crystallographica. Section D, Biological Crystallography·Ezgi Karaca, Alexandre M J J Bonvin
Aug 25, 2016·The Journal of Biological Chemistry·Edward A Dennis
Aug 20, 2019·International Journal of Biological Macromolecules·Marcus Vinícius Cardoso TrentoSilvana Marcussi
Sep 11, 1999·Toxicon : Official Journal of the International Society on Toxinology·S A AliW Voelter
Mar 10, 2010·Journal of Molecular Biology·Daniela DalmWinfried Hinrichs
Sep 10, 2002·Journal of Structural Biology·Hai-Long ZhangZheng-Jiong Lin
Mar 31, 2004·Biochemistry·Scott C BoegemanEdward A Dennis
Jul 17, 2009·Structure·Dariya S GlazerRuss B Altman

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