Structures of wild-type chloromet and L103N hydroxomet Themiste zostericola myohemerythrins at 1.8 A resolution

Biochemistry
L J MartinsW R Ellis

Abstract

Myohemerythrin (Mhr) is a nonheme iron oxygen carrier found in the retractor muscles of marine "peanut" worms. The X-ray crystal structures of two recombinant Themiste zostericola Mhrs are reported to a resolution of 1.8 A. Surprisingly, the met wild-type structure (R = 17.8%) was found to contain chloride bound to Fe2, while coordinated hydroxide was found in the met L103N structure (R = 18.3%). An internal water molecule was also found distal to the Fe-O-Fe center of the mutant protein, forming hydrogen bonds with the coordinated hydroxide and the OD1 atom of Asn-103. This finding is consistent with the kinetic and spectroscopic results reported for the L103N mutant Mhr [Raner, G. M., Martins, L. J., & Ellis, W. R., Jr. (1997) Biochemistry 36, 7037-7043]. Possible roles for the side chain of residue 103 (Leu in wild-type Mhr) in gating ligand binding are also discussed.

References

Apr 5, 1991·Journal of Molecular Biology·M A HolmesR E Stenkamp
Feb 1, 1989·Trends in Biochemical Sciences·M F Perutz
Sep 20, 1987·Journal of Molecular Biology·S SheriffJ L Smith
Jul 25, 1974·Journal of Molecular Biology·W A Hendrickson, G L Klippenstein
Aug 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S Kamtekar, M H Hecht

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Citations

Nov 7, 2000·Journal of Inorganic Biochemistry·C R LloydW R Ellis
Nov 28, 2014·Microbiology and Molecular Biology Reviews : MMBR·Kieran D CollinsKaren M Ottemann
Apr 5, 2000·The Journal of Biological Chemistry·C S FarmerJ Sanders-Loehr
Jul 29, 2019·Archives of Biochemistry and Biophysics·David S Weber, Jeffrey J Warren
May 16, 2021·Archives of Biochemistry and Biophysics·Maria Luiza Caldas NogueiraVictor L Davidson
Oct 24, 2001·Inorganic Chemistry·M. Tyler Caudle, Jeff W. Kampf

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