PMID: 11337144May 5, 2001Paper

Studies about the mechanism of internalization by mammary epithelial cells of Escherichia coli isolated from persistent bovine mastitis

Veterinary Microbiology
D DöpferW Gaastra

Abstract

The purpose of this study was to investigate the interaction between Escherichia coli and primary mammary epithelial cell cultures derived from cows with persistent intramammary infection (IMI). Two strains of E. coli, isolated from the milk of two different cows suffering from persistent E. coli IMI were tested for adhesion to and invasion of three primary mammary epithelial cell cultures derived from mammary biopsies of the two infected cows. Intracellular E. coli were detected during five days post infection in vitro. Both strains of E. coli adhered to and invaded monolayers of all three primary mammary epithelial cell cultures. One strain adhered less but invaded more than the other. Comparison with other mammary pathogens indicated that E. coli invaded the cells less efficiently than Staphylococcus aureus, about as efficiently as Streptococcus dysgalactiae and more efficiently than Streptococcus uberis. The mechanism of E. coli invasion was studied using the cytoskeleton disrupting agents colchicine and cytochalasin D. These compounds inhibited the invasion of E. coli. Invasion of E. coli could also be inhibited by the phosphokinase inhibitors genistein and staurosporin in a dose-dependent fashion. Phorbol-myristyl-acetate...Continue Reading

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Citations

Jan 24, 2004·Microbes and Infection·Margareta VerdrenghAndrej Tarkowski
Oct 1, 2011·Veterinary Immunology and Immunopathology·Ynte H SchukkenUNKNOWN members of the Pfizer mastitis research consortium
Feb 23, 2008·Current Opinion in Microbiology·Nahum Y ShpigelIlan Rosenshine
Jun 19, 2014·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Urszula L Burska, Jonathan N Fletcher
Sep 4, 2015·PloS One·Shlomo E BlumGabriel Leitner
Jun 6, 2012·Memórias do Instituto Oswaldo Cruz·Monica Cristina de SouzaAna Luiza de Mattos Guaraldi
Feb 27, 2008·Veterinary Microbiology·Samantha PasseyHarry Mellor
Sep 18, 2004·Journal of Dairy Science·F MoussaouiC Burvenich
Oct 3, 2002·The Veterinary Journal·A Bradley

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