PMID: 7371645Apr 1, 1980Paper

Studies by small-angle X-ray scattering of the quaternary structure of the 24-S component of the haemocyanin of Astacus leptodactylus in solution

European Journal of Biochemistry
I PilzR Lontie

Abstract

The haemocyanin of Astacus leptodactylus was studied in several buffers at three pH values. The best stability and lowest mean deviation on repeating the measurements were found at pH 7.2 in a Tris-HCl buffer. The following molecular parameters were determined: radius of gyration 6.90 nm, radius of gyration of the cross-section 3.87 nm, maximum dimension 21.5 nm, relative molecular mass 854000, volume 1440 nm3, hydration 0.27 g H2O/g protein. The theoretical scattering curves of a large number of models were calculated to find one fitting these data and the experimental scattering curve. The model with the best agreement was compared with an electron micrograph.

References

Jan 1, 1979·Methods in Enzymology·I PilzO Kratky
Jan 1, 1972·Clinica Chimica Acta; International Journal of Clinical Chemistry·A Isaacs
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J K Stoops, S J Wakil
Nov 15, 1963·Mycopathologia et mycologia applicata·G F ORRO A PLUNKETT

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Citations

Feb 20, 1990·Biotechnology and Bioengineering·M T Tyn, T W Gusek
Jan 1, 1983·Progress in Biophysics and Molecular Biology·H D EllertonH A Robinson
Jan 1, 1988·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·T T Herskovits
Nov 7, 2000·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·H Decker, R Föll
Mar 3, 1986·European Journal of Biochemistry·M Bijlholt, E F van Bruggen
Feb 1, 1982·Quarterly Reviews of Biophysics·K E van Holde, K I Miller
Sep 26, 2003·Biophysical Journal·Francesco SpinozziMariano Beltramini
Nov 1, 1983·Archives internationales de physiologie et de biochimie·M Durliat

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