PMID: 6406545May 1, 1983Paper

Studies in normal and chronic granulomatous disease neutrophils indicate a correlation of tubulin tyrosinolation with the cellular redox state

The Journal of Clinical Investigation
J Nath, J I Gallin

Abstract

A specific stimulation of tubulin tyrosinolation in human polymorphonuclear leukocytes (PMN) is induced by the synthetic peptide chemoattractant, N-formyl-methionyl-leucyl-phenylalanine (fMet-Leu-Phe), and this stimulation of tyrosinolation in PMN is completely inhibited in the presence of various reducing agents. Further studies to characterize the mechanism of stimulation of tyrosinolation in PMN have revealed that conditions that inhibited the respiratory burst in stimulated PMN, e.g., an anaerobic atmosphere, or addition of antioxidants such as cysteamine, azide, or 2,3-dihydroxybenzoic acid, also inhibited the peptide-induced stimulation of tyrosinolation in these cells. Moreover, the sulfhydryl reagent, N-ethylmaleimide, depressed tyrosinolation in resting PMN and completely inhibited the fMet-Leu-Phe-induced stimulation. In contrast, addition of diamide, which preferentially oxidizes cellular glutathione, significantly stimulated tyrosinolation both in resting and fMet-Leu-Phe-stimulated PMN. Furthermore, resting levels of tyrosinolation in seven patients with chronic granulomatous disease (CGD), whose oxidative metabolism is severely depressed, were 35-45% lower (P less than 0.01). Most strikingly, PMN from CGD patients...Continue Reading

References

Feb 1, 1977·The Journal of Pediatrics·L C McPhailC E McCall
Mar 23, 1978·The New England Journal of Medicine·B M Babior
Feb 1, 1977·The Journal of Clinical Investigation·I M GoldsteinH B Kaplan
Jan 1, 1979·The Journal of Clinical Investigation·B DewaldB M Babior
May 17, 1977·Biochemistry·D Raybin, M Flavin
Jul 1, 1976·The Journal of Clinical Investigation·J G ThoeneJ A Schneider
Oct 1, 1976·The Journal of Clinical Investigation·L C McPhailP S Shirley
Mar 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E SchiffmannS M Wahl
Mar 30, 1972·Biochimica Et Biophysica Acta·E M KosowerN S Kosower
May 2, 1968·The New England Journal of Medicine·R L Baehner, D G Nathan
May 1, 1982·Pediatric Research·P E Newburger

❮ Previous
Next ❯

Citations

Nov 15, 1988·Biochemical and Biophysical Research Communications·J Nath, A Powledge
May 20, 2003·The British Journal of Dermatology·A E C KiszewskiR Hernández Pando
Jul 12, 2002·Microscopy Research and Technique·Wolf D Splettstoesser, Peter Schuff-Werner
Jan 1, 1987·Annals of the New York Academy of Sciences·J Nath, J I Gallin
Jan 1, 1992·The British Journal of Nutrition·H Hemilä
Aug 1, 1991·Archives of Biochemistry and Biophysics·D B HinshawP A Hyslop

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.