Studies in red blood cell preservation: 9. The role of glutamine in red cell preservation

Vox Sanguinis
U J DumaswalaT J Greenwalt


Previous studies have demonstrated that an additive solution containing ammonium chloride (NH4+) and phosphate (Pi) in addition to adenine, glucose and mannitol would support red blood cell (RBC) in vitro characteristics and in vivo 24-hour viability after storage for 9 weeks. The purpose of the present study was to determine if NH4+ generated by the action of glutaminase on glutamine could be substituted for added NH4+ salts. Packed RBCs were stored with equal volumes of adenine, glucose, mannitol, and citrate containing additive solutions with 10 mM glutamine (EAS 31) or with 10 mM glutamine and either 10 (EAS 36) or 20 mM (EAS 37) Pi. One aliquot was stored with Adsol. The mean ATP levels of the RBCs stored in the glutamine plus phosphate EASs were 132 (10 mM Pi) and 144% (20 mM Pi) of the initial levels at 28 days, and at 84 days remained at 48 and 56%, respectively. The ATP levels of the RBC stored in Adsol were 105 and 25% at 28 and 84 days of storage, respectively. Percentage hemolysis and vesiculation was significantly lower (p < 0.01) for RBCs stored in glutamine and glutamine plus phosphate as compared to RBCs stored in Adsol. The levels of NH4+ were 22 to 34% higher in the EASs than in Adsol at the end of 84 days of ...Continue Reading


May 6, 1975·Biochimica Et Biophysica Acta·O Azzopardi, M F Jayle
Dec 15, 1992·Biochimica Et Biophysica Acta·N VincentG Baverel
Jan 1, 1990·Annales De Parasitologie Humaine Et Comparée·M VilmontF Frappier
Mar 30, 1990·European Journal of Biochemistry·D HäussingerW Gerok
Nov 1, 1986·Transfusion·H T MerymanR L Syring
Feb 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·A G PalekarA Meister
Nov 15, 1974·Molecular and Cellular Biochemistry·H M Kalckar
Feb 1, 1969·The Biochemical Journal·R C Jackson
May 1, 1970·Analytical Biochemistry·Z B Rose, J Liebowitz
Mar 1, 1966·Transfusion·E Beutler, O Duron
Nov 1, 1967·Transfusion·L Wood, E Beutler


Jan 21, 2012·Clinical Biochemistry·Mehmet Ramazan ŞekeroğluAydın Him
Nov 2, 2006·Transfusion·Alan TinmouthCanadian Critical Care Trials Group
Apr 9, 2001·Social Work in Health Care·M Dempster, M Donnelly
Mar 1, 1997·Transfusion·T J GreenwaltU J Dumaswala
Oct 3, 2020·BMC Pharmacology & Toxicology·Haozhen ShaoHongxuan Tong

Related Concepts

Ammonium Chloride
Blood Preservation
Anhydrous Dextrose
Phosphate-Activated Glutaminase
Hematocrit Procedure

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.