Dec 1, 1975

Studies in vitro of lipogenesis in rat testicular tissue

A R Whorton, J G Coniglio


Testicular tissue was shown to contain the full complement of enzymes required for de novo synthesis of fatty acids. The enzymes capable of snythesizing palmitic acid from citrate, acetate, or acetyl CoA were found to be present in the soluble (cytoplasmic) fraction. These included fatty acid synthetase, acetyl CoA carboxylase, citrate-cleavage enzyme, malic enzyme, glucose-6-phosphate dehydrogenase, and 6-phosphogluconate dehydrogenase. Optimal conditions for assaying activities of fatty acid synthetase and acetyl CoA carboxylase in the soluble fraction from rat testes were established, and the activities of these two enzymes were determined to be 0.54 +/- 0.1 and 0.030 +/- 0.002 (nmoles of substrate incorporated into fatty acid per min per mg of soluble fraction protein), respectively. The activities of citrate-cleavage enzyme, malic enzyme, and the glucose-6-phosphate dehydrogenase/6-phosphogluconate dehydrogenase pair were also measured. The activities were 6.0 +/- 0.7, 34.9 +/- 4.2, and 29.9 +/- 9.3 nmoles/min/mg, respectively.

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Mentioned in this Paper

Enzymes, antithrombotic
Malate dehydrogenase (decarboxylating)
Malate Dehydrogenase
Acetyl Coenzyme A
Cytokinesis of the Fertilized Ovum
Citrate Measurement
Glucosephosphate Dehydrogenase
Glucose-6-phosphate Dehydrogenase Measurement, Quantitative

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