Studies of antiproteolytic effects of glyburide on rat L6 myoblasts: comparisons with insulin

Metabolism: Clinical and Experimental
K C GorrayB S Schneider

Abstract

Cultured L6 myoblasts afford considerable advantages for identifying and studying the insulin-like actions of test substances in a muscle-derived line. We have used this system to examine the interaction of the oral hypoglycemic sulfonylurea glyburide with bovine insulin on protein degradation and synthesis as well as on thymidine incorporation (as a measure of DNA synthesis) in these cells. Bovine insulin, at doses of 0.1 microgram/mL to 10 micrograms/mL, produced a dose-dependent inhibition of protein degradation (measured by release of trichloracetic acid (TCA)-soluble 14C-tyrosine from myoblasts into the culture medium) and increase in total protein content in the cultured myoblasts. At concentrations of 10 micrograms/mL, insulin achieved its maximal suppression of protein degradation (by nearly 50%) and increased cellular protein content (by 15%) over levels observed in the absence of added insulin. Glyburide, at concentrations at or above 1 microgram/mL, significantly suppressed protein degradation (up to 14%) and slightly augmented protein content of the cells. The effects of glyburide on protein degradation were additive with those of submaximally but not maximally effective concentrations of insulin, suggesting a commo...Continue Reading

References

Feb 23, 1987·Life Sciences·J M FaganM E Tischler
Jul 1, 1987·The Journal of Clinical Investigation·R A Gelfand, E J Barrett
Apr 9, 1987·Nature·I C RobinsonL M Carlsson
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Jan 1, 1986·The Biochemical Journal·F J BallardJ C Wallace
Jan 1, 1984·Diabetes Care·S P MoodleyM Marivate
Jul 1, 1982·Clinics in Endocrinology and Metabolism·O G KoltermanJ M Olefsky

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Citations

Oct 30, 1992·Biochemical and Biophysical Research Communications·B S SchneiderR A Greenwald
Nov 24, 2018·Diabetes/metabolism Research and Reviews·Evangelia KalaitzoglouKathryn M Thrailkill

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