PMID: 640850Jan 1, 1978Paper

Studies of globin chain synthesis and globin mRNA content in a patient homozygous for hemoglobin Lepore

Hemoglobin
B G ForgetD Housman

Abstract

Globin chain synthesis and globin mRNA content were studied in blood cells of a patient homozygous for Hb Lepore. Peripheral blood cells incubated with tritiated leucine synthesized approximately 1.5 to 3% as many Lepore globin chains as alpha chains. Globin mRNA in peripheral blood cell RNA was assayed by molecular hybridization assays using human alpha and beta cDNA, and the results indicated the presence of approximately 1% to 2% as much beta-like mRNA (presumably deltabeta Lepore mRNA) as alpha mRNA. The amount of Lepore deltabeta chain mRNA in peripheral blood cells is therefore proportional to the amount of Lepore globin chain synthesis in the same cells. An incidental observation was the finding that peripheral blood cell RNA of this patient, at a time when she was being heavily transfused, contained substantially higher levels of beta-like mRNA (relative to alpha mRNA) than in subsequent studies. Cell-free translation of this mRNA however revealed that it contained authentic beta chain mRNA which must have been derived in some way from the transfused blood cells.

References

Jun 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·S OttolenghiC S Pitcher
Mar 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·B J Clarke, D Housman
Jul 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G F TempleY W Kan
Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·Y W KanH E Varmus
Oct 17, 1975·Science·R D BarrS Perry
Apr 1, 1976·British Journal of Haematology·J PritchardD J Weatherall
Feb 16, 1972·Nature: New Biology·J M WhiteJ Reeve
Sep 5, 1973·Nature: New Biology·A V RobertsY Ohta
Apr 14, 1972·Biochemical and Biophysical Research Communications·A V RobertsJ B Clegg

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Citations

Apr 1, 1979·The Journal of Clinical Investigation·F RamirezN Quattrin
Nov 1, 1993·American Journal of Hematology·D P SewardT R Kinney
Jan 1, 1979·Hemoglobin·M MarinucciF Mandelli
Jan 1, 1980·Annals of the New York Academy of Sciences·F ConconiC M Perrotta
Jul 5, 1983·Journal of Molecular Biology·J Ross, A Pizarro
Jan 1, 1978·American Journal of Hematology·G R HonigL N Vida
Jan 1, 1980·Annals of the New York Academy of Sciences·D TuanB G Forget

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