PMID: 51834Sep 15, 1975Paper

Studies of lymphocyte stimulation by intact tumor-cell and solubilized tumor antigen

International Journal of Cancer. Journal International Du Cancer
J H DeanL W Law

Abstract

BALB/c mice were rendered immune to syngeneic SV40-induced sarcoma by subcutaneous injection of mKSA-TU5 tissue-culture adapted cells. Spleen cells from immune mice were examined for tumor-cell neutralization in the Winn assay as well as in in vitro lymphocyte stimulation assays. A microculture (200 mul) lymphocyte stimulation (LS) assay utilizing immune spleen cells was employed with mixed lymphocyte/tumor-cell cultures (MLTC) and the papain crude soluble (CS) extracts from mKSA-TU5 cells. Specificity in the LS assay was determined using spleen cells from mice immune to other syngeneic tumors and by soluble antigenic preparation of normal BALB/c spleen cells. The Winn assay studies demonstrated that spleen cells from mKSA-sensitized mice neutralized mKSA tumor cells and this was corroborated by their resistance to direct tumor challenge. Positive lymphocyte transformation responses in MLTC were observed when mKSA-TU5-sensitized spleen cells were mixed with mitocycin-C-treated intact tumor cells or when papain-solubilized antigens of mKSA cells were employed, but not with non-immune spleen cells or with a soluble antigen from normal cells. Papain-solubilized antigen preparations employed in in vitro assays also immunized agains...Continue Reading

References

Jan 15, 1975·International Journal of Cancer. Journal International Du Cancer·R A Knight, R M Gorczynski
Jan 15, 1975·International Journal of Cancer. Journal International Du Cancer·M W BurkC F McKhann
Jan 15, 1975·International Journal of Cancer. Journal International Du Cancer·S B HowellL W Law
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Citations

Jan 1, 1976·Annals of the New York Academy of Sciences·L W LawM Rogers
Apr 1, 1983·Toxicology and Applied Pharmacology·M P DieterJ W Cox
Mar 30, 1987·Toxicology and Applied Pharmacology·G J Rosenthal, C A Snyder
Jul 1, 1979·Diseases of the Colon and Rectum·J J O'Connor
Nov 15, 1975·International Journal of Cancer. Journal International Du Cancer·J H DeanE Appella
Dec 15, 1977·International Journal of Cancer. Journal International Du Cancer·J H DeanL W Law
Feb 15, 1981·International Journal of Cancer. Journal International Du Cancer·G B CannonC Alford

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