PMID: 7028418Jan 1, 1981Paper

Studies of mammalian chromosome replication. II. Evidence for the existence of defined chromosome replicating units

Chromosoma
Y F Lau, F E Arrighi

Abstract

Sister chromatids of metaphase chromosomes can be differentially stained if the cells have replicated their DNA semiconservatively for two cell cycles in a medium containing 5-bromodeoxyuridine (BrdU). When prematurely condensed chromosomes (PCC) are induced in cells during the second S phase after BrdU is added to the medium, the replicated chromosome segments show sister chromatid differential (SCD) staining. Employing this PCC-SCD system on synchronous and asynchronous Chinese hamster ovary (CHO) cells, we have demonstrated that the replication patterns of the CHO cells can be categorized into G1/S, early, early-mid, mid-late, and late S phase patterns according to the amount of replicated chromosomes. During the first 4 h of the S phase, the replication patterns show SCD staining in chains of small chromosome segments. The amount of replicated chromosomes increase during the mid-late and late S categories (last 4 h). Significantly, small SCD segments are also present during these later intervals of the S phase. Measurements of these replicated segments indicate the presence of characteristic chromosome fragment sizes between 0.2 to 1.2 micrometers in all S phase cells except those at G1/S which contain no SCD fragments. The...Continue Reading

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Citations

May 10, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Michelle F Pflumm
Jun 1, 1989·Journal of Molecular Evolution·G P Holmquist
Nov 1, 1982·Cell·G HolmquistJ Jordan
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Dec 16, 1998·The Journal of Cell Biology·H MaR Berezney
Jul 1, 2006·Journal of Cellular Physiology·Eisuke Gotoh, Marco Durante
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Nov 13, 1986·Biochimica Et Biophysica Acta·G P Holmquist, L A Caston
Nov 3, 1989·Science·L H Hartwell, T A Weinert

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